Abstract
The class I region of the human leukocyte antigen (HLA) complex includes genes encoding the classical transplantation antigens (HLA-A, -B, -C), at least three nonclassical class I genes (HLA-E, -F, and -G), and many class I pseudogenes (including HLA-7.5p). We have used probes from DNA within or flanking the HLA -A, -F, -G, and -7.5p genes to construct a physical linkage map that places the HLA-F, -G, and -7.5p loci in order with respect to HLA-A. The map was constructed using clamped homogeneous electric field pulsed-field gel electrophoresis. DNA was isolated from LCL 721 (A1:B8, A2:B5), a human Epstein-Barr virus-transformed lymphoblastoid cell line (LCL), and from two γ-irradiation-induced mutants of LCL 721 lacking complementary class I haplotypes. The physical linkage data place HLA-G closest to HLA-A and place HLA-7.5p between HLA-G and HLA-F. The map constructed supports a maximum distance of 490 kilobases between HLA-A and HLA-F. Human Immunology 31, 180-185 (1991).
| Original language | English (US) |
|---|---|
| Pages (from-to) | 180-185 |
| Number of pages | 6 |
| Journal | Human Immunology |
| Volume | 31 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1991 |
Bibliographical note
Funding Information:We thank S.K. Bronson and Dr. D.D. Chaplin fi~r helpful discussions of unpublished data. We also thank Dr. S. Scherer and members of his laboratory for providing yeast cultures and protocols for the preparation of yeast chromosomes in agarose blocks, and Dr. J. Perentisis fi}r assisting in the identification of the largest S. cervisiae chromosomes. Support for these studies was provided by Grant AI 18124 from the N IH.