A perfused rabbit retina preparation suitable for pharmacological studies

Robert F. Miller; Robert A. Zalutsky; Stephen C. Massey

doi:10.1016/0165-0270(86)90056-7

A perfused rabbit retina preparation suitable for pharmacological studies

Robert F. Miller, Robert A. Zalutsky, Stephen C. Massey

Neuroscience

Research output: Contribution to journal › Article › peer-review

24 Scopus citations

Abstract

We present a method for maintaining the isolated retina-eyecup of the rabbit in a manner which permits the introduction of pharmacological agents at a controlled concentration. As judged by physiological criteria, the retina is well maintained under these conditions. The stability of the preparation is excellent; intracellular or extracellular recordings from single cells can be maintained through multiple solution changes. By using Co²⁺ to block synaptic transmission, we can distinguish direct from transsynaptic effects. Use of this preparation should facilitate the investigation of neurotransmission in the mammalian retina.

Original language	English (US)
Pages (from-to)	309-322
Number of pages	14
Journal	Journal of Neuroscience Methods
Volume	16
Issue number	4
DOIs	https://doi.org/10.1016/0165-0270(86)90056-7
State	Published - Jun 1986

Bibliographical note

Funding Information:
This research was supported by NEI grants EY03014 (R.F.M.) and EY05766 (S.C.M.). We wish to thank Mr. Art Reel and Ms. Judy Dodge for excellent technical assistance and Dr. A.I. Cohen and Dr. C. Blazynski for their comments on the manuscript.

Keywords

cobalt
electrophysiology
perfusion
rabbit retina
synaptic block

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10.1016/0165-0270(86)90056-7

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title = "A perfused rabbit retina preparation suitable for pharmacological studies",

abstract = "We present a method for maintaining the isolated retina-eyecup of the rabbit in a manner which permits the introduction of pharmacological agents at a controlled concentration. As judged by physiological criteria, the retina is well maintained under these conditions. The stability of the preparation is excellent; intracellular or extracellular recordings from single cells can be maintained through multiple solution changes. By using Co2+ to block synaptic transmission, we can distinguish direct from transsynaptic effects. Use of this preparation should facilitate the investigation of neurotransmission in the mammalian retina.",

keywords = "cobalt, electrophysiology, perfusion, rabbit retina, synaptic block",

author = "Miller, {Robert F.} and Zalutsky, {Robert A.} and Massey, {Stephen C.}",

note = "Funding Information: This research was supported by NEI grants EY03014 (R.F.M.) and EY05766 (S.C.M.). We wish to thank Mr. Art Reel and Ms. Judy Dodge for excellent technical assistance and Dr. A.I. Cohen and Dr. C. Blazynski for their comments on the manuscript.",

year = "1986",

month = jun,

doi = "10.1016/0165-0270(86)90056-7",

language = "English (US)",

volume = "16",

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TY - JOUR

T1 - A perfused rabbit retina preparation suitable for pharmacological studies

AU - Miller, Robert F.

AU - Zalutsky, Robert A.

AU - Massey, Stephen C.

N1 - Funding Information: This research was supported by NEI grants EY03014 (R.F.M.) and EY05766 (S.C.M.). We wish to thank Mr. Art Reel and Ms. Judy Dodge for excellent technical assistance and Dr. A.I. Cohen and Dr. C. Blazynski for their comments on the manuscript.

PY - 1986/6

Y1 - 1986/6

N2 - We present a method for maintaining the isolated retina-eyecup of the rabbit in a manner which permits the introduction of pharmacological agents at a controlled concentration. As judged by physiological criteria, the retina is well maintained under these conditions. The stability of the preparation is excellent; intracellular or extracellular recordings from single cells can be maintained through multiple solution changes. By using Co2+ to block synaptic transmission, we can distinguish direct from transsynaptic effects. Use of this preparation should facilitate the investigation of neurotransmission in the mammalian retina.

AB - We present a method for maintaining the isolated retina-eyecup of the rabbit in a manner which permits the introduction of pharmacological agents at a controlled concentration. As judged by physiological criteria, the retina is well maintained under these conditions. The stability of the preparation is excellent; intracellular or extracellular recordings from single cells can be maintained through multiple solution changes. By using Co2+ to block synaptic transmission, we can distinguish direct from transsynaptic effects. Use of this preparation should facilitate the investigation of neurotransmission in the mammalian retina.

KW - cobalt

KW - electrophysiology

KW - perfusion

KW - rabbit retina

KW - synaptic block

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DO - 10.1016/0165-0270(86)90056-7

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AN - SCOPUS:0022472313

SN - 0165-0270

VL - 16

SP - 309

EP - 322

JO - Journal of Neuroscience Methods

JF - Journal of Neuroscience Methods

IS - 4

ER -

A perfused rabbit retina preparation suitable for pharmacological studies

Abstract

Bibliographical note

Keywords

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