Sucrose is the main saccharide used for long-distance transport in plants and plays an essential role in energy metabolism; however, there are no analogues for real-time imaging in live cells. We have optimised a synthetic approach to prepare sucrose analogues including very small (≈50 Da or less) Raman tags in the fructose moiety. Spectroscopic analysis identified the alkyne-tagged compound 6 as a sucrose analogue recognised by endogenous transporters in live cells and with higher Raman intensity than other sucrose derivatives. Herein, we demonstrate the application of compound 6 as the first optical probe to visualise real-time uptake and intracellular localisation of sucrose in live plant cells using Raman microscopy.
Bibliographical noteFunding Information:
The authors acknowledge funding from the Biotechnology and Biological Sciences Research Council (BB/M025160/1), the Medical Research Council (MR/R01566X/1), Cancer Research UK (C157/A25140 and C157/A15703), the Office of Basic Energy Sciences of the U.S. Department of Energy (DE‐FG02‐07ER15886) and the University of Strathclyde. M.V. acknowledges funds from an ERC Consolidator Grant (771443).
© 2021 The Authors. Angewandte Chemie International Edition published by Wiley-VCH GmbH
- stimulated Raman scattering
PubMed: MeSH publication types
- Journal Article
- Research Support, Non-U.S. Gov't
- Research Support, U.S. Gov't, Non-P.H.S.