A novel yeast genomic DNA library on a geneticin-resistance vector

Peter A. Jauert, Linnea E. Jensen, David T Kirkpatrick

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

We have constructed a Saccharomyces cerevisiae genomic library utilizing a novel centromere-containing vector bearing the TRP1 and KANMX4 G418-resistance selectable markers. Random Sau3AI genomic DNA fragments with an average size of 6.4 kb were introduced into a unique BamHI site in the vector's polylinker. Six independent library pools were generated and the characteristics of these pools were determined. This library can be used to introduce low-copy genomic DNA fragments into any strain that is not G418-resistant, making it useful for low-copy overexpression analysis or complementation of mutant phenotypes in strains that lack a suitable auxotrophy marker or only exhibit phenotypes on rich, undefined, growth media.

Original languageEnglish (US)
Pages (from-to)653-657
Number of pages5
JournalYeast
Volume22
Issue number8
DOIs
StatePublished - Jun 2005

Keywords

  • Complementation
  • Drug Resistance
  • Library
  • Overexpression

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