Abstract
We have constructed a Saccharomyces cerevisiae genomic library utilizing a novel centromere-containing vector bearing the TRP1 and KANMX4 G418-resistance selectable markers. Random Sau3AI genomic DNA fragments with an average size of 6.4 kb were introduced into a unique BamHI site in the vector's polylinker. Six independent library pools were generated and the characteristics of these pools were determined. This library can be used to introduce low-copy genomic DNA fragments into any strain that is not G418-resistant, making it useful for low-copy overexpression analysis or complementation of mutant phenotypes in strains that lack a suitable auxotrophy marker or only exhibit phenotypes on rich, undefined, growth media.
Original language | English (US) |
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Pages (from-to) | 653-657 |
Number of pages | 5 |
Journal | Yeast |
Volume | 22 |
Issue number | 8 |
DOIs | |
State | Published - Jun 2005 |
Keywords
- Complementation
- Drug Resistance
- Library
- Overexpression