A novel method of determining the number of macromolecules per asymmetric unit from accurate crystal-volume measurements

Fang Li, Thomas A. Steitz

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Knowledge of the number of macromolecules per crystallographic asymmetric unit is frequently useful in the determination of crystal structures. A method has been developed to establish this number directly from measurements of the volume and macromolecular contents of a crystal. The volume of a crystal is determined by measuring the volume of solvent that it displaces in a fine capillary tube. The macromolecular mass contained in a crystal is measured by dissolving the crystal in a known amount of water or suitable buffer and then measuring the UV absorbance of the solution. The method has been tested successfully on three different crystals of known structures.

Original languageEnglish (US)
Pages (from-to)1793-1796
Number of pages4
JournalActa Crystallographica - Section D Biological Crystallography
Volume59
Issue number10
DOIs
StatePublished - Oct 1 2003

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