A novel glutamate-dependent acid resistance among strains belonging to the Proteeae tribe of Enterobacteriaceae

Geun Woo Park, Francisco Diez-Gonzalez

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Morganella, Providencia and Proteus strains were capable of surviving pH 2.0 for 1 h if glutamate was present. These strains did not have glutamic acid decarboxylase activity and the gadAB genes were not detected in any of these bacteria. When exposed to pH 2.0 acid shocks, the survival rate of these bacteria was significantly increased with glutamate concentrations as low as 0.3 mM in the acid media. Escherichia coli cells incubated at pH 3.4 consumed four times more glutamate and produced at least 7-fold more 7gamma;-amino butyric acid than Morganella, Providencia and Proteus strains. These results indicate that strains belonging to the Proteeae tribe might have novel glutamate dependent acid-resistance mechanisms.

Original languageEnglish (US)
Pages (from-to)303-309
Number of pages7
JournalFEMS Microbiology Letters
Volume237
Issue number2
DOIs
StatePublished - Aug 15 2004

Fingerprint

Enterobacteriaceae
Glutamic Acid
Morganella
Providencia
Proteus
Acids
Bacteria
Glutamate Decarboxylase
Butyric Acid
Shock
Escherichia coli
Genes

Keywords

  • Acid Resistance
  • Escherichia coli
  • Glutamate Decarboxylase
  • Morganella
  • Proteus
  • Providencia

Cite this

A novel glutamate-dependent acid resistance among strains belonging to the Proteeae tribe of Enterobacteriaceae. / Park, Geun Woo; Diez-Gonzalez, Francisco.

In: FEMS Microbiology Letters, Vol. 237, No. 2, 15.08.2004, p. 303-309.

Research output: Contribution to journalArticle

@article{62cd1c01560d478880b5479efbb97909,
title = "A novel glutamate-dependent acid resistance among strains belonging to the Proteeae tribe of Enterobacteriaceae",
abstract = "Morganella, Providencia and Proteus strains were capable of surviving pH 2.0 for 1 h if glutamate was present. These strains did not have glutamic acid decarboxylase activity and the gadAB genes were not detected in any of these bacteria. When exposed to pH 2.0 acid shocks, the survival rate of these bacteria was significantly increased with glutamate concentrations as low as 0.3 mM in the acid media. Escherichia coli cells incubated at pH 3.4 consumed four times more glutamate and produced at least 7-fold more 7gamma;-amino butyric acid than Morganella, Providencia and Proteus strains. These results indicate that strains belonging to the Proteeae tribe might have novel glutamate dependent acid-resistance mechanisms.",
keywords = "Acid Resistance, Escherichia coli, Glutamate Decarboxylase, Morganella, Proteus, Providencia",
author = "Park, {Geun Woo} and Francisco Diez-Gonzalez",
year = "2004",
month = "8",
day = "15",
doi = "10.1016/j.femsle.2004.06.050",
language = "English (US)",
volume = "237",
pages = "303--309",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Wiley-Blackwell",
number = "2",

}

TY - JOUR

T1 - A novel glutamate-dependent acid resistance among strains belonging to the Proteeae tribe of Enterobacteriaceae

AU - Park, Geun Woo

AU - Diez-Gonzalez, Francisco

PY - 2004/8/15

Y1 - 2004/8/15

N2 - Morganella, Providencia and Proteus strains were capable of surviving pH 2.0 for 1 h if glutamate was present. These strains did not have glutamic acid decarboxylase activity and the gadAB genes were not detected in any of these bacteria. When exposed to pH 2.0 acid shocks, the survival rate of these bacteria was significantly increased with glutamate concentrations as low as 0.3 mM in the acid media. Escherichia coli cells incubated at pH 3.4 consumed four times more glutamate and produced at least 7-fold more 7gamma;-amino butyric acid than Morganella, Providencia and Proteus strains. These results indicate that strains belonging to the Proteeae tribe might have novel glutamate dependent acid-resistance mechanisms.

AB - Morganella, Providencia and Proteus strains were capable of surviving pH 2.0 for 1 h if glutamate was present. These strains did not have glutamic acid decarboxylase activity and the gadAB genes were not detected in any of these bacteria. When exposed to pH 2.0 acid shocks, the survival rate of these bacteria was significantly increased with glutamate concentrations as low as 0.3 mM in the acid media. Escherichia coli cells incubated at pH 3.4 consumed four times more glutamate and produced at least 7-fold more 7gamma;-amino butyric acid than Morganella, Providencia and Proteus strains. These results indicate that strains belonging to the Proteeae tribe might have novel glutamate dependent acid-resistance mechanisms.

KW - Acid Resistance

KW - Escherichia coli

KW - Glutamate Decarboxylase

KW - Morganella

KW - Proteus

KW - Providencia

UR - http://www.scopus.com/inward/record.url?scp=4143121083&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4143121083&partnerID=8YFLogxK

U2 - 10.1016/j.femsle.2004.06.050

DO - 10.1016/j.femsle.2004.06.050

M3 - Article

VL - 237

SP - 303

EP - 309

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 2

ER -