A novel ankyrin-repeat protein interacts with the regulatory proteins of inner arm dynein f (I1) of Chlamydomonas reinhardtii

Kazuho Ikeda, Ryosuke Yamamoto, Maureen Wirschell, Toshiki Yagi, Raqual Bower, Mary E Porter, Winfield S. Sale, Ritsu Kamiya

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

How ciliary and flagellar motility is regulated is a challenging problem. The flagellar movement in Chlamydomonas reinhardtii is in part regulated by phosphorylation of a 138 kD intermediate chain (IC138) of inner arm dynein f (also called I1). In the present study, we found that the axoneme of mutants lacking dynein f lacks a novel protein having ankyrin repeat motifs, registered as FAP120 in the flagellar proteome database. FAP120 is also missing or decreased in the axonemes of bop5, a mutant that has a mutation in the structural gene of IC138 but assembles the dynein f complex. Intriguingly, the amounts of FAP120 in the axonemes of different alleles of bop5 and several dynein f-lacking mutants roughly parallel their contents of IC138. These results suggest a weak but stoichiometric interaction between FAP120 and IC138. We propose that FAP120 functions in the regulatory process as part of a protein complex involving IC138.

Original languageEnglish (US)
Pages (from-to)448-456
Number of pages9
JournalCell motility and the cytoskeleton
Volume66
Issue number8
DOIs
StatePublished - Aug 1 2009

Fingerprint

Ankyrin Repeat
Chlamydomonas reinhardtii
Dyneins
Axoneme
Proteins
Proteome
Alleles
Phosphorylation
Databases
Mutation
Genes

Keywords

  • Cilia
  • Dynein f(I1)
  • FAP120
  • IC138
  • IC97

Cite this

A novel ankyrin-repeat protein interacts with the regulatory proteins of inner arm dynein f (I1) of Chlamydomonas reinhardtii. / Ikeda, Kazuho; Yamamoto, Ryosuke; Wirschell, Maureen; Yagi, Toshiki; Bower, Raqual; Porter, Mary E; Sale, Winfield S.; Kamiya, Ritsu.

In: Cell motility and the cytoskeleton, Vol. 66, No. 8, 01.08.2009, p. 448-456.

Research output: Contribution to journalArticle

Ikeda, Kazuho ; Yamamoto, Ryosuke ; Wirschell, Maureen ; Yagi, Toshiki ; Bower, Raqual ; Porter, Mary E ; Sale, Winfield S. ; Kamiya, Ritsu. / A novel ankyrin-repeat protein interacts with the regulatory proteins of inner arm dynein f (I1) of Chlamydomonas reinhardtii. In: Cell motility and the cytoskeleton. 2009 ; Vol. 66, No. 8. pp. 448-456.
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