A nondenatured, noncrosslinked collagen matrix to deliver stem cells to the heart

Nicholas A. Kouris, Jayne M. Squirrell, Jangwook P. Jung, Carolyn A. Pehlke, Timothy Hacker, Kevin W. Eliceiri, Brenda M. Ogle

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Aims: Stem cell transplantation holds promise as a therapeutic approach for the repair of damaged myocardial tissue. One challenge of this approach is efficient delivery and long-term retention of the stem cells. Although several synthetic and natural biomaterials have been developed for this purpose, the ideal formulation has yet to be identified. Materials & methods: Here we investigate the utility of a nondenatured, noncrosslinked, commercially available natural biomaterial (TissueMend® [TEI Biosciences, Boston, MA, USA]) for delivery of human mesenchymal stem cells (MSCs) to the murine heart. Results: We found that MSCs attached, proliferated and migrated within and out of the TissueMend matrix in vitro. Human MSCs delivered to damaged murine myocardium via the matrix (2.3 × 10 4 ± 0.8 × 10 4 CD73 + cells/matrix) were maintained in vivo for 3 weeks and underwent at least three population doublings during that period (21.9 × 10 4 ± 14.4 × 10 4 CD73 + cells/matrix). In addition, collagen within the TissueMend matrix could be remodeled by MSCs in vivo, resulting in a significant decrease in the coefficient of alignment of fibers (0.12 ± 0.12) compared with the matrix alone (0.28 ± 0.07), and the MSCs were capable of migrating out of the matrix and into the host tissue. Conclusion: Thus, TissueMend matrix offers a commercially available, biocompatible and malleable vehicle for the delivery and retention of stem cells to the heart.

Original languageEnglish (US)
Pages (from-to)569-582
Number of pages14
JournalRegenerative Medicine
Volume6
Issue number5
DOIs
StatePublished - Sep 1 2011

Fingerprint

Stem cells
Mesenchymal Stromal Cells
Collagen
Stem Cells
Biocompatible Materials
Biomaterials
Stem Cell Transplantation
Tissue
Myocardium
Repair
Population
Fibers
Therapeutics

Keywords

  • biomaterial
  • cardiac patch
  • cardiac regeneration
  • collagen
  • mesenchymal stem cells

Cite this

Kouris, N. A., Squirrell, J. M., Jung, J. P., Pehlke, C. A., Hacker, T., Eliceiri, K. W., & Ogle, B. M. (2011). A nondenatured, noncrosslinked collagen matrix to deliver stem cells to the heart. Regenerative Medicine, 6(5), 569-582. https://doi.org/10.2217/rme.11.48

A nondenatured, noncrosslinked collagen matrix to deliver stem cells to the heart. / Kouris, Nicholas A.; Squirrell, Jayne M.; Jung, Jangwook P.; Pehlke, Carolyn A.; Hacker, Timothy; Eliceiri, Kevin W.; Ogle, Brenda M.

In: Regenerative Medicine, Vol. 6, No. 5, 01.09.2011, p. 569-582.

Research output: Contribution to journalArticle

Kouris, NA, Squirrell, JM, Jung, JP, Pehlke, CA, Hacker, T, Eliceiri, KW & Ogle, BM 2011, 'A nondenatured, noncrosslinked collagen matrix to deliver stem cells to the heart', Regenerative Medicine, vol. 6, no. 5, pp. 569-582. https://doi.org/10.2217/rme.11.48
Kouris NA, Squirrell JM, Jung JP, Pehlke CA, Hacker T, Eliceiri KW et al. A nondenatured, noncrosslinked collagen matrix to deliver stem cells to the heart. Regenerative Medicine. 2011 Sep 1;6(5):569-582. https://doi.org/10.2217/rme.11.48
Kouris, Nicholas A. ; Squirrell, Jayne M. ; Jung, Jangwook P. ; Pehlke, Carolyn A. ; Hacker, Timothy ; Eliceiri, Kevin W. ; Ogle, Brenda M. / A nondenatured, noncrosslinked collagen matrix to deliver stem cells to the heart. In: Regenerative Medicine. 2011 ; Vol. 6, No. 5. pp. 569-582.
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