A noncatalytic function of the topoisomerase II CTD in Aurora B recruitment to inner centromeres during mitosis

Heather Edgerton, Marnie Johansson, Daniel Keifenheim, Soumya Mukherjee, Jeremy M. Chacón, Jeff Bachant, Melissa K. Gardner, Duncan J. Clarke

Research output: Contribution to journalArticlepeer-review

20 Scopus citations


Faithful chromosome segregation depends on the precise timing of chromatid separation, which is enforced by checkpoint signals generated at kinetochores. Here, we provide evidence that the C-terminal domain (CTD) of DNA topoisomerase IIα (Topo II) provides a novel function at inner centromeres of kinetochores in mitosis. We find that the yeast CTD is required for recruitment of the tension checkpoint kinase Ipl1/Aurora B to inner centromeres in metaphase but is not required in interphase. Conserved CTD SUMOylation sites are required for Ipl1 recruitment. This inner-centromere CTD function is distinct from the catalytic activity of Topo II. Genetic and biochemical evidence suggests that Topo II recruits Ipl1 via the Haspin-histone H3 threonine 3 phosphorylation pathway. Finally, Topo II and Sgo1 are equally important for Ipl1 recruitment to inner centromeres. This indicates H3 T3-Phos/H2A T120-Phos is a universal epigenetic signature that defines the eukaryotic inner centromere and provides the binding site for Ipl1/Aurora B.

Original languageEnglish (US)
Pages (from-to)651-664
Number of pages14
JournalJournal of Cell Biology
Issue number6
StatePublished - 2016

Bibliographical note

Funding Information:
Funding was provided by National Institutes of Health grant GM112793. J. Bachant acknowledges funding from the California Cancer Research Coordinating Committee that contributed to this work.

Publisher Copyright:
© 2016 Edgerton et al.

Copyright 2017 Elsevier B.V., All rights reserved.

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