Abstract
Faithful chromosome segregation depends on the precise timing of chromatid separation, which is enforced by checkpoint signals generated at kinetochores. Here, we provide evidence that the C-terminal domain (CTD) of DNA topoisomerase IIα (Topo II) provides a novel function at inner centromeres of kinetochores in mitosis. We find that the yeast CTD is required for recruitment of the tension checkpoint kinase Ipl1/Aurora B to inner centromeres in metaphase but is not required in interphase. Conserved CTD SUMOylation sites are required for Ipl1 recruitment. This inner-centromere CTD function is distinct from the catalytic activity of Topo II. Genetic and biochemical evidence suggests that Topo II recruits Ipl1 via the Haspin-histone H3 threonine 3 phosphorylation pathway. Finally, Topo II and Sgo1 are equally important for Ipl1 recruitment to inner centromeres. This indicates H3 T3-Phos/H2A T120-Phos is a universal epigenetic signature that defines the eukaryotic inner centromere and provides the binding site for Ipl1/Aurora B.
Original language | English (US) |
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Pages (from-to) | 651-664 |
Number of pages | 14 |
Journal | Journal of Cell Biology |
Volume | 213 |
Issue number | 6 |
DOIs | |
State | Published - 2016 |
Bibliographical note
Funding Information:Funding was provided by National Institutes of Health grant GM112793. J. Bachant acknowledges funding from the California Cancer Research Coordinating Committee that contributed to this work.
Publisher Copyright:
© 2016 Edgerton et al.