Abstract
A culture medium has been formulated to support the long-term proliferation and differentiation of normal murine epidermal cells in primary cultures. This formulation is based upon MCDB-151 modified by the addition of 1.2 mM Ca2+, 1% fetal bovine serum (FBS) or 0.1% purified bovine serum albumin (BSA), trace elements, and growth supplements. For 4-12 weeks, the cultures demonstrate desmosomes, keratin fibers, basal proliferation, and stratification characteristic of keratinocytes. The addition of 12-O-tetradecanoylphorbol-13-acetate (TPA) resulted in approximately 70% cellular detachment, but with continued treatment, the remaining cells proliferated to confluence and stratified. This culture medium has application to a number of studies related to skin carcinogenesis in the murine model system that have not previously been possible.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 297-304 |
| Number of pages | 8 |
| Journal | Cancer Letters |
| Volume | 34 |
| Issue number | 3 |
| DOIs | |
| State | Published - Mar 1987 |
| Externally published | Yes |
Bibliographical note
Funding Information:*Submitted in part in abstract form for poster presentation at the Amer. Assoc. Cancer Res. meeting May 1986. This study was supported by U.S. Public Health Service grants CA-34443 (SMF), CA-34962 (TJS) and CA-34521 (TJS). **To whom requests for reprints should be sent.