TY - JOUR
T1 - A new cultivation method for microbial oil production
T2 - Cell pelletization and lipid accumulation by Mucor circinelloides
AU - Xia, Chunjie
AU - Zhang, Jianguo
AU - Zhang, Weidong
AU - Hu, Bo
N1 - Funding Information:
CX’s study at the University of Minnesota was partly supported by the Special Fund for studying abroad from Beijing University of Chemical Technology, and the research was supported by BH’s faculty seed money program at the University of Minnesota.
PY - 2011
Y1 - 2011
N2 - The recent energy crisis has triggered significant attention on the microbial synthesis of lipids, which comprise the raw material for biodiesel production. Microbial oil accumulation with filamentous fungi has great potential because filamentous fungi can form pellets during cell growth, and these pellets are much easier to harvest from cell broth. This paper focuses on the cell pelletization process of the oleaginous Mucor circinelloides. We have studied the effect of various cultural conditions on pelletized cell growth and lipid accumulation. This study is the first to report that pH adjustment during cell growth plays a key role in pellet formation of M. circinelloides and describes a handy method by which to induce cell pelletization in submerged fungal cultivation. Our study reveals that cell growth and lipid production are not significantly affected by pelletization and that lipid accumulation is triggered at stressed conditions, such as a high carbon-to-nitrogen ratio and high temperature.
AB - The recent energy crisis has triggered significant attention on the microbial synthesis of lipids, which comprise the raw material for biodiesel production. Microbial oil accumulation with filamentous fungi has great potential because filamentous fungi can form pellets during cell growth, and these pellets are much easier to harvest from cell broth. This paper focuses on the cell pelletization process of the oleaginous Mucor circinelloides. We have studied the effect of various cultural conditions on pelletized cell growth and lipid accumulation. This study is the first to report that pH adjustment during cell growth plays a key role in pellet formation of M. circinelloides and describes a handy method by which to induce cell pelletization in submerged fungal cultivation. Our study reveals that cell growth and lipid production are not significantly affected by pelletization and that lipid accumulation is triggered at stressed conditions, such as a high carbon-to-nitrogen ratio and high temperature.
UR - http://www.scopus.com/inward/record.url?scp=79957825469&partnerID=8YFLogxK
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U2 - 10.1186/1754-6834-4-15
DO - 10.1186/1754-6834-4-15
M3 - Article
C2 - 21635739
AN - SCOPUS:79957825469
SN - 1754-6834
VL - 4
JO - Biotechnology for Biofuels
JF - Biotechnology for Biofuels
M1 - 15
ER -
