A new betasatellite associated with cotton leaf curl Burewala virus infecting tomato in India: Influence on symptoms and viral accumulation

Jitendra Kumar, Samatha Gunapati, Sudhir P. Singh, Abhinav Kumar, Adarsh Lalit, Naresh C. Sharma, Rekha Puranik, Rakesh Tuli

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

A begomovirus and its associated alpha- and betasatellite were detected in tomato plants affected with leaf curl disease. Based on a nucleotide sequence identity of 99 %, this begomovirus was designated an isolate of cotton leaf curl Burewala virus (CLCuBuV). The alphasatellite exhibited 93 % sequence identity to cotton leaf curl Burewala alphasatellite (CLCuBuA) and is hence referred to here as a variant of CLCuBuA. The detected betasatellite was recombinant in nature and showed 70 % sequence identity to the known betasatellites. Inoculation of healthy tomato with CLCuBuV plus betasatellite, either in the presence or the absence of alphasatellite, led to typical leaf curling, while inoculation with CLCuBuV in the absence of betasatellite resulted in mild symptoms. This confirmed the role of the betasatellite in expression of disease symptoms. We propose to name the newly detected betasatellite tomato leaf curl Hajipur betasatellite (ToLCHJB).

Original languageEnglish (US)
Pages (from-to)1349-1353
Number of pages5
JournalArchives of Virology
Volume158
Issue number6
DOIs
StatePublished - Jun 1 2013

Keywords

  • Alphasatellite
  • Begomovirus
  • Betasatellite
  • Geminivirus
  • Recombination

Fingerprint Dive into the research topics of 'A new betasatellite associated with cotton leaf curl Burewala virus infecting tomato in India: Influence on symptoms and viral accumulation'. Together they form a unique fingerprint.

Cite this