A multiplex RT-PCR assay for the detection of fish picornaviruses

Sunil K. Mor, Nicholas B.D. Phelps, Marisa Barbknecht, Michael A. Hoffman, Sagar M. Goyal

Research output: Contribution to journalArticlepeer-review

2 Scopus citations


With the emergence of high profile fish diseases in the Great Lakes region, surveillance and regulatory inspections of fish populations have increased. This has resulted in a better understanding of known pathogens and isolation of many new pathogens of fish. In this study, a multiplex RT-PCR assay was developed for the detection of three newly discovered fish picornaviruses: bluegill picornavirus-1 (BGPV-1), fathead minnow picornavirus (FHMPV), and eel picornavirus-1 (EPV-1). This assay was found to be very sensitive with a detection limit of 81.9. pg/μl of extracted RNA from a pool of FHMPV and BGPV-1 and was able to detect 501 and 224 gene copies/μl of BGPV-1 and FHMPV, respectively. The assay was highly reproducible and did not cross react with other closely related pathogens. We believe that this new assay provides a rapid and cost effective tool for confirming cell culture isolates and conducting prevalence studies of these newly detected fish picornaviruses.

Original languageEnglish (US)
Pages (from-to)131-134
Number of pages4
JournalJournal of Virological Methods
StatePublished - 2015

Bibliographical note

Publisher Copyright:
© 2015 Elsevier B.V..


  • Bluegill picornavirus
  • Eel picornavirus
  • Fathead minnow picornavirus
  • Multiplex RT-PCR
  • Reproducibility
  • Sensitivity
  • Specificity


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