A multiparametric fluorescence approach for biomembrane studies

Ahmed A. Heikal

doi:10.1016/B978-0-12-387721-5.00005-5

A multiparametric fluorescence approach for biomembrane studies

Ahmed A. Heikal

Chemistry & Biochemistry

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Biological membranes are heterogeneous assemblies of a variety of lipids and proteins as well as cholesterol. The dynamic nature of these biomembranes spans a wide range of spatiotemporal scales that are essential to their function in cell signaling and biomolecular trafficking. In contrast, biomimetic membranes are simple, stable, and versatile systems to study the physicochemical principles such as lipid mixing, phase separation, domain formation and intermolecular interactions, which underlie lipid bilayers assemblies under controlled conditions. In this chapter, multiparametric fluorescence micro-spectroscopy approach will be described for quantitative and noninvasive investigation of molecular processes that trigger lateral and temporal heterogeneities in biomembranes.

Original language	English (US)
Pages (from-to)	169-197
Number of pages	29
Journal	Advances in Planar Lipid Bilayers and Liposomes
Volume	13
DOIs	https://doi.org/10.1016/B978-0-12-387721-5.00005-5
State	Published - May 19 2011

Keywords

Fluorescence correlation spectroscopy
Fluorescence lifetime imaging microscopy
Giant unilamellar vesicles
Liquid domains
Time-resolved anisotropy
Translational diffusion

Access

10.1016/B978-0-12-387721-5.00005-5

Fingerprint Dive into the research topics of 'A multiparametric fluorescence approach for biomembrane studies'. Together they form a unique fingerprint.

Cite this

@article{d70d14b518cd4f4e86f5b9abbd54b517,

title = "A multiparametric fluorescence approach for biomembrane studies",

abstract = "Biological membranes are heterogeneous assemblies of a variety of lipids and proteins as well as cholesterol. The dynamic nature of these biomembranes spans a wide range of spatiotemporal scales that are essential to their function in cell signaling and biomolecular trafficking. In contrast, biomimetic membranes are simple, stable, and versatile systems to study the physicochemical principles such as lipid mixing, phase separation, domain formation and intermolecular interactions, which underlie lipid bilayers assemblies under controlled conditions. In this chapter, multiparametric fluorescence micro-spectroscopy approach will be described for quantitative and noninvasive investigation of molecular processes that trigger lateral and temporal heterogeneities in biomembranes.",

keywords = "Fluorescence correlation spectroscopy, Fluorescence lifetime imaging microscopy, Giant unilamellar vesicles, Liquid domains, Time-resolved anisotropy, Translational diffusion",

author = "Heikal, {Ahmed A.}",

year = "2011",

month = may,

day = "19",

doi = "10.1016/B978-0-12-387721-5.00005-5",

language = "English (US)",

volume = "13",

pages = "169--197",

journal = "Advances in Biomembranes and Lipid Self-Assembly",

issn = "2451-9634",

publisher = "Elsevier BV",

}

TY - JOUR

T1 - A multiparametric fluorescence approach for biomembrane studies

AU - Heikal, Ahmed A.

PY - 2011/5/19

Y1 - 2011/5/19

N2 - Biological membranes are heterogeneous assemblies of a variety of lipids and proteins as well as cholesterol. The dynamic nature of these biomembranes spans a wide range of spatiotemporal scales that are essential to their function in cell signaling and biomolecular trafficking. In contrast, biomimetic membranes are simple, stable, and versatile systems to study the physicochemical principles such as lipid mixing, phase separation, domain formation and intermolecular interactions, which underlie lipid bilayers assemblies under controlled conditions. In this chapter, multiparametric fluorescence micro-spectroscopy approach will be described for quantitative and noninvasive investigation of molecular processes that trigger lateral and temporal heterogeneities in biomembranes.

AB - Biological membranes are heterogeneous assemblies of a variety of lipids and proteins as well as cholesterol. The dynamic nature of these biomembranes spans a wide range of spatiotemporal scales that are essential to their function in cell signaling and biomolecular trafficking. In contrast, biomimetic membranes are simple, stable, and versatile systems to study the physicochemical principles such as lipid mixing, phase separation, domain formation and intermolecular interactions, which underlie lipid bilayers assemblies under controlled conditions. In this chapter, multiparametric fluorescence micro-spectroscopy approach will be described for quantitative and noninvasive investigation of molecular processes that trigger lateral and temporal heterogeneities in biomembranes.

KW - Fluorescence correlation spectroscopy

KW - Fluorescence lifetime imaging microscopy

KW - Giant unilamellar vesicles

KW - Liquid domains

KW - Time-resolved anisotropy

KW - Translational diffusion

UR - http://www.scopus.com/inward/record.url?scp=79955952218&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79955952218&partnerID=8YFLogxK

U2 - 10.1016/B978-0-12-387721-5.00005-5

DO - 10.1016/B978-0-12-387721-5.00005-5

M3 - Article

AN - SCOPUS:79955952218

VL - 13

SP - 169

EP - 197

JO - Advances in Biomembranes and Lipid Self-Assembly

JF - Advances in Biomembranes and Lipid Self-Assembly

SN - 2451-9634

ER -