A monoclonal antibody to ICP4 of MDV recognizing ICP4 of serotype 1 and 3 MDV strains

Z. Xing; Q. Xie; R. W. Morgan; K. A. Schat

A monoclonal antibody to ICP4 of MDV recognizing ICP4 of serotype 1 and 3 MDV strains

Z. Xing, Q. Xie, R. W. Morgan, K. A. Schat

Veterinary and Biomedical Sciences

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Monoclonal antibodies (MAbs) were prepared against ICP4 of Marek's disease virus (MDV). Mice were inoculated with ICP4 obtained from High-Five insect cells infected with a recombinant baculovirus expressing ICP4. MAbs were selected by enzyme-linked immunosorbent assay (ELISA) using MDV-infected and control chick kidney cells as antigens. One of the MAbs, 5H8, recognized an epitope toward the carboxyl terminus of ICP4 based on staining of reticuloendotheliosis virus-transformed cells transfected with full-length and truncated ICP4 constructs. This MAb recognized ICP4 in chicken embryo fibroblasts (CEFs) infected with MDV strains JM 16 and HVT but not with SB-1 strain. Using Western blot analysis a protein of 155 kDa was detected in CEFs infected with JM 16 and HVT strains.

Original language	English (US)
Pages (from-to)	113-120
Number of pages	8
Journal	Acta Virologica
Volume	43
Issue number	2-3
State	Published - Apr 1 1999

Keywords

ICP4
MDV
Monoclonal antibody

Find It

Find It at U of M Libraries

Cite this

@article{06bb57ede8934e758857977c2fae25a7,

title = "A monoclonal antibody to ICP4 of MDV recognizing ICP4 of serotype 1 and 3 MDV strains",

abstract = "Monoclonal antibodies (MAbs) were prepared against ICP4 of Marek's disease virus (MDV). Mice were inoculated with ICP4 obtained from High-Five insect cells infected with a recombinant baculovirus expressing ICP4. MAbs were selected by enzyme-linked immunosorbent assay (ELISA) using MDV-infected and control chick kidney cells as antigens. One of the MAbs, 5H8, recognized an epitope toward the carboxyl terminus of ICP4 based on staining of reticuloendotheliosis virus-transformed cells transfected with full-length and truncated ICP4 constructs. This MAb recognized ICP4 in chicken embryo fibroblasts (CEFs) infected with MDV strains JM 16 and HVT but not with SB-1 strain. Using Western blot analysis a protein of 155 kDa was detected in CEFs infected with JM 16 and HVT strains.",

keywords = "ICP4, MDV, Monoclonal antibody",

author = "Z. Xing and Q. Xie and Morgan, {R. W.} and Schat, {K. A.}",

year = "1999",

month = apr,

day = "1",

language = "English (US)",

volume = "43",

pages = "113--120",

journal = "Acta Virologica",

issn = "0001-723X",

publisher = "AEP - Academic Electronic Press Ltd.",

number = "2-3",

}

TY - JOUR

T1 - A monoclonal antibody to ICP4 of MDV recognizing ICP4 of serotype 1 and 3 MDV strains

AU - Xing, Z.

AU - Xie, Q.

AU - Morgan, R. W.

AU - Schat, K. A.

PY - 1999/4/1

Y1 - 1999/4/1

N2 - Monoclonal antibodies (MAbs) were prepared against ICP4 of Marek's disease virus (MDV). Mice were inoculated with ICP4 obtained from High-Five insect cells infected with a recombinant baculovirus expressing ICP4. MAbs were selected by enzyme-linked immunosorbent assay (ELISA) using MDV-infected and control chick kidney cells as antigens. One of the MAbs, 5H8, recognized an epitope toward the carboxyl terminus of ICP4 based on staining of reticuloendotheliosis virus-transformed cells transfected with full-length and truncated ICP4 constructs. This MAb recognized ICP4 in chicken embryo fibroblasts (CEFs) infected with MDV strains JM 16 and HVT but not with SB-1 strain. Using Western blot analysis a protein of 155 kDa was detected in CEFs infected with JM 16 and HVT strains.

AB - Monoclonal antibodies (MAbs) were prepared against ICP4 of Marek's disease virus (MDV). Mice were inoculated with ICP4 obtained from High-Five insect cells infected with a recombinant baculovirus expressing ICP4. MAbs were selected by enzyme-linked immunosorbent assay (ELISA) using MDV-infected and control chick kidney cells as antigens. One of the MAbs, 5H8, recognized an epitope toward the carboxyl terminus of ICP4 based on staining of reticuloendotheliosis virus-transformed cells transfected with full-length and truncated ICP4 constructs. This MAb recognized ICP4 in chicken embryo fibroblasts (CEFs) infected with MDV strains JM 16 and HVT but not with SB-1 strain. Using Western blot analysis a protein of 155 kDa was detected in CEFs infected with JM 16 and HVT strains.

KW - ICP4

KW - MDV

KW - Monoclonal antibody

UR - http://www.scopus.com/inward/record.url?scp=0033033333&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033033333&partnerID=8YFLogxK

M3 - Article

C2 - 10696430

AN - SCOPUS:0033033333

SN - 0001-723X

VL - 43

SP - 113

EP - 120

JO - Acta Virologica

JF - Acta Virologica

IS - 2-3

ER -

A monoclonal antibody to ICP4 of MDV recognizing ICP4 of serotype 1 and 3 MDV strains

Abstract

Keywords

Find It

Other files and links

Fingerprint

Cite this