Abstract
Monoclonal antibodies (MAbs) were prepared against ICP4 of Marek's disease virus (MDV). Mice were inoculated with ICP4 obtained from High-Five insect cells infected with a recombinant baculovirus expressing ICP4. MAbs were selected by enzyme-linked immunosorbent assay (ELISA) using MDV-infected and control chick kidney cells as antigens. One of the MAbs, 5H8, recognized an epitope toward the carboxyl terminus of ICP4 based on staining of reticuloendotheliosis virus-transformed cells transfected with full-length and truncated ICP4 constructs. This MAb recognized ICP4 in chicken embryo fibroblasts (CEFs) infected with MDV strains JM 16 and HVT but not with SB-1 strain. Using Western blot analysis a protein of 155 kDa was detected in CEFs infected with JM 16 and HVT strains.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 113-120 |
| Number of pages | 8 |
| Journal | Acta Virologica |
| Volume | 43 |
| Issue number | 2-3 |
| State | Published - Apr 1999 |
Keywords
- ICP4
- MDV
- Monoclonal antibody
