Abstract
We have produced a monoclonal antibody that specifically recognizes the adrenocorticotropic receptor on rat adrenal cells. The immunogen was designed from an RNA sequence complementary to the mRNA coding for ACTH1-24. This complementary peptide, termed HTCA, has been shown to specifically bind ACTH and was proposed to mimic the ACTH binding site of the hormone receptor. The monoclonal anti-HTCA antibody recognized a restricted domain of the HTCA peptide, bound to Y-1 adrenal cells with a KD of 1.8 nM, and blocked the binding of 125I-ACTH to rat adrenal cells. These findings show that anti-HTCA competes with ACTH for binding to the ACTH receptor.
Original language | English (US) |
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Pages (from-to) | 1020-1026 |
Number of pages | 7 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 168 |
Issue number | 3 |
DOIs | |
State | Published - May 16 1990 |
Bibliographical note
Funding Information:Acknowledgments. We would like to preparation of this manuscript, Todd assistance, and David Pascual for insightful supported by NIDDKD grant DK 39299.