A molecular and phylogenetic analysis of the structure and specificity of Alnus rubra ectomycorrhizal assemblages

Peter G. Kennedy; Lee T. Hill

doi:10.1016/j.funeco.2009.08.005

A molecular and phylogenetic analysis of the structure and specificity of Alnus rubra ectomycorrhizal assemblages

Peter G. Kennedy, Lee T. Hill

Research output: Contribution to journal › Article › peer-review

32 Scopus citations

Abstract

Ectomycorrhizal (EM) assemblages associated with Alnus spp. are often distinct in composition and richness from other host plants. To examine the EM assemblage associated with A. rubra, a common tree in western coastal North America, we sampled four A. rubra forests varying in age, management history, and geographic location in Oregon, USA. From the 364 EM root tip rDNA ITS sequences, we found a total of 14 EM taxa. The five most abundant taxa, Tomentella sp. 3, Alnicola escharoides, Tomentella sp. 1, Lactarius cf. obscuratus, and Alpova diplophloeus, represented 80. % of the samples and were present at all four sites. Assemblage structure differed significantly among young managed sites and older unmanaged sites but not by geographic location. The younger managed sites had higher tree density, Frankia frequency, and soil nitrogen than older unmanaged sites. Phylogenetic analyses revealed that Alnus-associated EM congeners were not closely related, indicating the distinct nature of Alnus EM assemblages is not due to a unique co-evolutionary history.

Original language	English (US)
Pages (from-to)	195-204
Number of pages	10
Journal	Fungal Ecology
Volume	3
Issue number	3
DOIs	https://doi.org/10.1016/j.funeco.2009.08.005
State	Published - Aug 2010
Externally published	Yes

Bibliographical note

Funding Information:
The authors thank Andrew Bluhm and David Hibbs for assistance with use of the HSC study sites, J. Yahya for assistance with the Frankia frequency analysis, Joe Ammirati, Greta Binford, Ursula Eberhardt, Brandon Matheny, Martin Ryberg, Andy Taylor, and Else Vellinga for assistance with the phylogenetic analyses. Many members of the Lewis and Clark College Biology department, Martha Palomino, Kabir Peay, Marjorie Weber, and two reviewers provided constructive comments on a previous version of this manuscript. Funding was provided by the Murdock Charitable Trust, the Lewis & Clark College Student Academic Affairs Board, and the National Science Foundation (DEB 0742868 to P. Kennedy and T. Bruns).

Keywords

Alnus rubra
Assemblage structure
Ectomycorrhiza
Host specificity
Pacific Northwest

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10.1016/j.funeco.2009.08.005

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title = "A molecular and phylogenetic analysis of the structure and specificity of Alnus rubra ectomycorrhizal assemblages",

abstract = "Ectomycorrhizal (EM) assemblages associated with Alnus spp. are often distinct in composition and richness from other host plants. To examine the EM assemblage associated with A. rubra, a common tree in western coastal North America, we sampled four A. rubra forests varying in age, management history, and geographic location in Oregon, USA. From the 364 EM root tip rDNA ITS sequences, we found a total of 14 EM taxa. The five most abundant taxa, Tomentella sp. 3, Alnicola escharoides, Tomentella sp. 1, Lactarius cf. obscuratus, and Alpova diplophloeus, represented 80. % of the samples and were present at all four sites. Assemblage structure differed significantly among young managed sites and older unmanaged sites but not by geographic location. The younger managed sites had higher tree density, Frankia frequency, and soil nitrogen than older unmanaged sites. Phylogenetic analyses revealed that Alnus-associated EM congeners were not closely related, indicating the distinct nature of Alnus EM assemblages is not due to a unique co-evolutionary history.",

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author = "Kennedy, {Peter G.} and Hill, {Lee T.}",

note = "Funding Information: The authors thank Andrew Bluhm and David Hibbs for assistance with use of the HSC study sites, J. Yahya for assistance with the Frankia frequency analysis, Joe Ammirati, Greta Binford, Ursula Eberhardt, Brandon Matheny, Martin Ryberg, Andy Taylor, and Else Vellinga for assistance with the phylogenetic analyses. Many members of the Lewis and Clark College Biology department, Martha Palomino, Kabir Peay, Marjorie Weber, and two reviewers provided constructive comments on a previous version of this manuscript. Funding was provided by the Murdock Charitable Trust, the Lewis & Clark College Student Academic Affairs Board, and the National Science Foundation (DEB 0742868 to P. Kennedy and T. Bruns). ",

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N2 - Ectomycorrhizal (EM) assemblages associated with Alnus spp. are often distinct in composition and richness from other host plants. To examine the EM assemblage associated with A. rubra, a common tree in western coastal North America, we sampled four A. rubra forests varying in age, management history, and geographic location in Oregon, USA. From the 364 EM root tip rDNA ITS sequences, we found a total of 14 EM taxa. The five most abundant taxa, Tomentella sp. 3, Alnicola escharoides, Tomentella sp. 1, Lactarius cf. obscuratus, and Alpova diplophloeus, represented 80. % of the samples and were present at all four sites. Assemblage structure differed significantly among young managed sites and older unmanaged sites but not by geographic location. The younger managed sites had higher tree density, Frankia frequency, and soil nitrogen than older unmanaged sites. Phylogenetic analyses revealed that Alnus-associated EM congeners were not closely related, indicating the distinct nature of Alnus EM assemblages is not due to a unique co-evolutionary history.

AB - Ectomycorrhizal (EM) assemblages associated with Alnus spp. are often distinct in composition and richness from other host plants. To examine the EM assemblage associated with A. rubra, a common tree in western coastal North America, we sampled four A. rubra forests varying in age, management history, and geographic location in Oregon, USA. From the 364 EM root tip rDNA ITS sequences, we found a total of 14 EM taxa. The five most abundant taxa, Tomentella sp. 3, Alnicola escharoides, Tomentella sp. 1, Lactarius cf. obscuratus, and Alpova diplophloeus, represented 80. % of the samples and were present at all four sites. Assemblage structure differed significantly among young managed sites and older unmanaged sites but not by geographic location. The younger managed sites had higher tree density, Frankia frequency, and soil nitrogen than older unmanaged sites. Phylogenetic analyses revealed that Alnus-associated EM congeners were not closely related, indicating the distinct nature of Alnus EM assemblages is not due to a unique co-evolutionary history.

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A molecular and phylogenetic analysis of the structure and specificity of Alnus rubra ectomycorrhizal assemblages

Abstract

Bibliographical note

Keywords

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