A molecular and morphological analysis of the genus Rhizopogon subgenus Villosuli section Villosuli as a preface to ecological monitoring

Ryan Bubriski, Peter G Kennedy

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Rhizopogon (Boletales) represents a model genus for ecological studies of ectomycorrhizal fungi, but the identification of species in subgenus Villosuli section Villosuli has long been challenging due to variation in taxonomically informative morphological characters. Here we re-examine species concepts in this section using data from nuclear ribosomal internal transcribed spacer (ITS) and large subunit (LSU) sequences as well as spore measurements. Our phylogenetic analyses from 34 type and non-type collections of eight species, including the seven currently recognized in this section, found consistent support for only three species-level clades. Each of the clades had a significantly different combination of mean spore widths and Q ratios using digitalassisted measurements, which suggests both molecular and spore-based morphological approaches can be used to identify species in this section. Based on our analysis, we propose that only three species names be applied to future ecological studies: R. hawkerae, R. parksii and R. villosulus. We consider R. subareolatus and R. colossus as taxonomic synonyms of R. hawkerae and R. pseudovillosulus, R. rogersii, R. villescens and R. zelleri as taxonomic synonyms of R. villosulus.

Original languageEnglish (US)
Pages (from-to)353-361
Number of pages9
JournalMycologia
Volume106
Issue number2
DOIs
StatePublished - Jan 1 2014

Fingerprint

Rhizopogon
Spores
spores
monitoring
spore
Boletales
internal transcribed spacers
Names
Fungi
species concept
fungi
phylogeny
analysis
fungus
phylogenetics

Keywords

  • Ecology
  • ITS
  • LSU
  • Rhizopogon
  • Spore size
  • Taxonomy
  • Villosuli

Cite this

A molecular and morphological analysis of the genus Rhizopogon subgenus Villosuli section Villosuli as a preface to ecological monitoring. / Bubriski, Ryan; Kennedy, Peter G.

In: Mycologia, Vol. 106, No. 2, 01.01.2014, p. 353-361.

Research output: Contribution to journalArticle

@article{fc3e189ec0db4c6a8ba6822175819f7d,
title = "A molecular and morphological analysis of the genus Rhizopogon subgenus Villosuli section Villosuli as a preface to ecological monitoring",
abstract = "Rhizopogon (Boletales) represents a model genus for ecological studies of ectomycorrhizal fungi, but the identification of species in subgenus Villosuli section Villosuli has long been challenging due to variation in taxonomically informative morphological characters. Here we re-examine species concepts in this section using data from nuclear ribosomal internal transcribed spacer (ITS) and large subunit (LSU) sequences as well as spore measurements. Our phylogenetic analyses from 34 type and non-type collections of eight species, including the seven currently recognized in this section, found consistent support for only three species-level clades. Each of the clades had a significantly different combination of mean spore widths and Q ratios using digitalassisted measurements, which suggests both molecular and spore-based morphological approaches can be used to identify species in this section. Based on our analysis, we propose that only three species names be applied to future ecological studies: R. hawkerae, R. parksii and R. villosulus. We consider R. subareolatus and R. colossus as taxonomic synonyms of R. hawkerae and R. pseudovillosulus, R. rogersii, R. villescens and R. zelleri as taxonomic synonyms of R. villosulus.",
keywords = "Ecology, ITS, LSU, Rhizopogon, Spore size, Taxonomy, Villosuli",
author = "Ryan Bubriski and Kennedy, {Peter G}",
year = "2014",
month = "1",
day = "1",
doi = "10.3852/106.2.353",
language = "English (US)",
volume = "106",
pages = "353--361",
journal = "Mycologia",
issn = "0027-5514",
publisher = "Allen Press Inc.",
number = "2",

}

TY - JOUR

T1 - A molecular and morphological analysis of the genus Rhizopogon subgenus Villosuli section Villosuli as a preface to ecological monitoring

AU - Bubriski, Ryan

AU - Kennedy, Peter G

PY - 2014/1/1

Y1 - 2014/1/1

N2 - Rhizopogon (Boletales) represents a model genus for ecological studies of ectomycorrhizal fungi, but the identification of species in subgenus Villosuli section Villosuli has long been challenging due to variation in taxonomically informative morphological characters. Here we re-examine species concepts in this section using data from nuclear ribosomal internal transcribed spacer (ITS) and large subunit (LSU) sequences as well as spore measurements. Our phylogenetic analyses from 34 type and non-type collections of eight species, including the seven currently recognized in this section, found consistent support for only three species-level clades. Each of the clades had a significantly different combination of mean spore widths and Q ratios using digitalassisted measurements, which suggests both molecular and spore-based morphological approaches can be used to identify species in this section. Based on our analysis, we propose that only three species names be applied to future ecological studies: R. hawkerae, R. parksii and R. villosulus. We consider R. subareolatus and R. colossus as taxonomic synonyms of R. hawkerae and R. pseudovillosulus, R. rogersii, R. villescens and R. zelleri as taxonomic synonyms of R. villosulus.

AB - Rhizopogon (Boletales) represents a model genus for ecological studies of ectomycorrhizal fungi, but the identification of species in subgenus Villosuli section Villosuli has long been challenging due to variation in taxonomically informative morphological characters. Here we re-examine species concepts in this section using data from nuclear ribosomal internal transcribed spacer (ITS) and large subunit (LSU) sequences as well as spore measurements. Our phylogenetic analyses from 34 type and non-type collections of eight species, including the seven currently recognized in this section, found consistent support for only three species-level clades. Each of the clades had a significantly different combination of mean spore widths and Q ratios using digitalassisted measurements, which suggests both molecular and spore-based morphological approaches can be used to identify species in this section. Based on our analysis, we propose that only three species names be applied to future ecological studies: R. hawkerae, R. parksii and R. villosulus. We consider R. subareolatus and R. colossus as taxonomic synonyms of R. hawkerae and R. pseudovillosulus, R. rogersii, R. villescens and R. zelleri as taxonomic synonyms of R. villosulus.

KW - Ecology

KW - ITS

KW - LSU

KW - Rhizopogon

KW - Spore size

KW - Taxonomy

KW - Villosuli

UR - http://www.scopus.com/inward/record.url?scp=84901345333&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84901345333&partnerID=8YFLogxK

U2 - 10.3852/106.2.353

DO - 10.3852/106.2.353

M3 - Article

C2 - 24782502

AN - SCOPUS:84901345333

VL - 106

SP - 353

EP - 361

JO - Mycologia

JF - Mycologia

SN - 0027-5514

IS - 2

ER -