A module located at a chromosomal integration hot spot is responsible for the multidrug resistance of a reference strain from Escherichia coli clonal group A

Mathilde Lescat, Alexandra Calteau, Claire Hoede, Valérie Barbe, Marie Touchon, Eduardo Rocha, Olivier Tenaillon, Claudine Médigue, James R. Johnson, Erick Denamur

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Escherichia coli clonal group A (CGA) commonly exhibits a distinctive multidrug antimicrobial resistance phenotype - i.e., resistance to ampicillin, chloramphenicol, streptomycin, sulfonamides, tetracycline, and trimethoprim (ACSSuTTp) - and has accounted for up to 50% of trimethoprim-sulfamethoxazole- resistant E. coli urinary tract infections in some locales. Annotation of the whole-genome sequencing of UMN026, a reference CGA strain, clarified the genetic basis for this strain's ACSSuTTp antimicrobial resistance phenotype. Most of the responsible genes were clustered in a unique 23-kbp chromosomal region, designated the genomic resistance module (GRM), which occurred within a 105-kbp genomic island situated at the leuX tRNA. The GRM is characterized by numerous remnants of mobilization and rearrangement events suggesting multiple horizontal transfers. Additionally, comparative genomic analysis of the leuX tRNA genomic island in 14 sequenced E. coli genomes showed that this region is a hot spot of integration, with the presence/absence of specific subregions being uncorrelated with either the phylogenetic group or the pathotype. Our data illustrate the importance of whole-genome sequencing in the detection of genetic elements involved in antimicrobial resistance. Additionally, this is the first documentation of the blaTEM and dhfrVII genes in a chromosomal location in E. coli strains.

Original languageEnglish (US)
Pages (from-to)2283-2288
Number of pages6
JournalAntimicrobial agents and chemotherapy
Volume53
Issue number6
DOIs
StatePublished - Jun 2009

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Copyright 2018 Elsevier B.V., All rights reserved.

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