A modified enzyme-linked immunosorbent assay for the detection of avian pneumovirus antibodies

Shu Ju Chiang; Arshud M. Dar; Sagar M. Goyal; Mohammad A. Sheikh; Jan C. Pedersen; B. Panigrahy; Dennis Senne; David A. Halvorson; Kakambi V. Nagaraja; Vivek Kapur

doi:10.1177/104063870001200417

A modified enzyme-linked immunosorbent assay for the detection of avian pneumovirus antibodies

Shu Ju Chiang, Arshud M. Dar, Sagar M. Goyal, Mohammad A. Sheikh, Jan C. Pedersen, B. Panigrahy, Dennis Senne, David A. Halvorson, Kakambi V. Nagaraja, Vivek Kapur

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Abstract

Avian pneumovirus (APV) infection of turkeys in Minnesota was first confirmed in March 1997. Serum samples (n = 5,194) from 539 submissions to Minnesota Veterinary Diagnostic Laboratory were tested by a modified enzyme-linked immunosorbent assay (ELISA). Of these, 2,528 (48.7%) samples from 269 submissions were positive and 2,666 (51.3%) samples from 270 submissions were negative for APV antibodies. Most positive samples were from Kandiyohi, Stearns, Morrison, and Meeker counties in Minnesota. In addition, 10 samples from South Dakota were positive. The sensitivity and specificity of the ELISA test with anti-chicken and anti-turkey conjugates were compared by testing field and experimental sera. The ELISA test with anti-turkey conjugate was more sensitive than that with anti-chicken conjugate. The ELISA tests with antigens prepared with APV strains isolated from Colorado and Minnesota were also compared. No difference was detectable. Currently, the Minnesota Veterinary Diagnostic Laboratory uses an antigen prepared from the Colorado isolate of APV and a goat anti-turkey conjugate in the ELISA test.

Original language	English (US)
Pages (from-to)	381-384
Number of pages	4
Journal	Journal of Veterinary Diagnostic Investigation
Volume	12
Issue number	4
DOIs	https://doi.org/10.1177/104063870001200417
State	Published - Jul 2000

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title = "A modified enzyme-linked immunosorbent assay for the detection of avian pneumovirus antibodies",

abstract = "Avian pneumovirus (APV) infection of turkeys in Minnesota was first confirmed in March 1997. Serum samples (n = 5,194) from 539 submissions to Minnesota Veterinary Diagnostic Laboratory were tested by a modified enzyme-linked immunosorbent assay (ELISA). Of these, 2,528 (48.7%) samples from 269 submissions were positive and 2,666 (51.3%) samples from 270 submissions were negative for APV antibodies. Most positive samples were from Kandiyohi, Stearns, Morrison, and Meeker counties in Minnesota. In addition, 10 samples from South Dakota were positive. The sensitivity and specificity of the ELISA test with anti-chicken and anti-turkey conjugates were compared by testing field and experimental sera. The ELISA test with anti-turkey conjugate was more sensitive than that with anti-chicken conjugate. The ELISA tests with antigens prepared with APV strains isolated from Colorado and Minnesota were also compared. No difference was detectable. Currently, the Minnesota Veterinary Diagnostic Laboratory uses an antigen prepared from the Colorado isolate of APV and a goat anti-turkey conjugate in the ELISA test.",

author = "Chiang, {Shu Ju} and Dar, {Arshud M.} and Goyal, {Sagar M.} and Sheikh, {Mohammad A.} and Pedersen, {Jan C.} and B. Panigrahy and Dennis Senne and Halvorson, {David A.} and Nagaraja, {Kakambi V.} and Vivek Kapur",

year = "2000",

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doi = "10.1177/104063870001200417",

language = "English (US)",

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pages = "381--384",

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AU - Chiang, Shu Ju

AU - Dar, Arshud M.

AU - Goyal, Sagar M.

AU - Sheikh, Mohammad A.

AU - Pedersen, Jan C.

AU - Panigrahy, B.

AU - Senne, Dennis

AU - Halvorson, David A.

AU - Nagaraja, Kakambi V.

AU - Kapur, Vivek

PY - 2000/7

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N2 - Avian pneumovirus (APV) infection of turkeys in Minnesota was first confirmed in March 1997. Serum samples (n = 5,194) from 539 submissions to Minnesota Veterinary Diagnostic Laboratory were tested by a modified enzyme-linked immunosorbent assay (ELISA). Of these, 2,528 (48.7%) samples from 269 submissions were positive and 2,666 (51.3%) samples from 270 submissions were negative for APV antibodies. Most positive samples were from Kandiyohi, Stearns, Morrison, and Meeker counties in Minnesota. In addition, 10 samples from South Dakota were positive. The sensitivity and specificity of the ELISA test with anti-chicken and anti-turkey conjugates were compared by testing field and experimental sera. The ELISA test with anti-turkey conjugate was more sensitive than that with anti-chicken conjugate. The ELISA tests with antigens prepared with APV strains isolated from Colorado and Minnesota were also compared. No difference was detectable. Currently, the Minnesota Veterinary Diagnostic Laboratory uses an antigen prepared from the Colorado isolate of APV and a goat anti-turkey conjugate in the ELISA test.

AB - Avian pneumovirus (APV) infection of turkeys in Minnesota was first confirmed in March 1997. Serum samples (n = 5,194) from 539 submissions to Minnesota Veterinary Diagnostic Laboratory were tested by a modified enzyme-linked immunosorbent assay (ELISA). Of these, 2,528 (48.7%) samples from 269 submissions were positive and 2,666 (51.3%) samples from 270 submissions were negative for APV antibodies. Most positive samples were from Kandiyohi, Stearns, Morrison, and Meeker counties in Minnesota. In addition, 10 samples from South Dakota were positive. The sensitivity and specificity of the ELISA test with anti-chicken and anti-turkey conjugates were compared by testing field and experimental sera. The ELISA test with anti-turkey conjugate was more sensitive than that with anti-chicken conjugate. The ELISA tests with antigens prepared with APV strains isolated from Colorado and Minnesota were also compared. No difference was detectable. Currently, the Minnesota Veterinary Diagnostic Laboratory uses an antigen prepared from the Colorado isolate of APV and a goat anti-turkey conjugate in the ELISA test.

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A modified enzyme-linked immunosorbent assay for the detection of avian pneumovirus antibodies

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