A model of cryosurgical destruction in AT-1 prostate tumor based on cellular damage mechanisms

David J. Smith, Sean J. Josephson, John C. Bischof

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Scopus citations

Abstract

The thermal history during a prostate cryosurgery is known to lead to different cooling rates, end-temperatures and end-times within a cryosurgical iceball. The tissue exposed to this range of thermal histories will experience thermally-induced biophysical events which affect cell viability (dehydration and intracellular ice formation, IIF), injury due solely to the temperature and time of exposure, and injury due to host response. In this study, the dehydration and IIF behavior of single AT-1 prostate cancer cells is experimentally measured, the biophysical parameters of water transport and IIF are obtained, and the probability of injury to single AT-1 cells due to dehydration (Prd) and IIF (PIF) is predicted for a variety of cryosurgically-relevant thermal histories. In addition, viability data obtained after cooling to different end-temperatures at constant cooling rates is used to create an empirical function of injury for single AT-1 cells based solely on end-temperature (Pre). A total cellular injury model which then combines the biophysical mechanisms of injury as well as the empirically-obtained temperature viability function is created. This model is used to predict worst-case (i.e. highest possible survival) cryosurgical destruction in an AT-1 tumor; the implications for clinical cryosurgery are discussed.

Original languageEnglish (US)
Title of host publicationAmerican Society of Mechanical Engineers, Bioengineering Division (Publication) BED
Pages149-150
Number of pages2
Volume37
StatePublished - 1997

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