A mixed valence form of the iron cluster in the B2 protein of ribonucleotide reductase from Escherichia coli

Michael P. Hendrich, Timothy E. Elgren, Lawrence Que

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Abstract

A mixed valent form of the iron cluster (Fe(II)Fe(III)) in the B2 protein of ribonucleotide reductase has been isolated and characterized. The irons in this state of the protein are ferromagnetically coupled as indicated by the observation of a novel S = 9/2 EPR spectrum. This is the first ferromagnetically coupled Fe(II)Fe(III) cluster reported for a protein and the first observation of the mixed valence form of ribonucleotide reductase.

Original languageEnglish (US)
Pages (from-to)705-710
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume176
Issue number2
DOIs
StatePublished - Apr 30 1991

Bibliographical note

Funding Information:
This work was supported by grants from the National Institute of Health, GM-12996 (M. P. H.), and from the National Science Foundation, DMB-8314935, DMB-8804458 (L. Q.). We thank Professor Joanne Stubbe for providing E. coli strain N6405/pSPS2 used in these experiments.

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