A Microtiter Modification of Granulocyte Immunofluorescence

Abstract. The granulocyte immunofluorescence test (GIFT) is valuable for detecting allogeneic and autologous granulocyte antibodies. However, the original tube technique (macro GIFT) requires 50–100 μl of serum and 10⁶ granulocytes with time‐consuming washing steps which cause cell loss. We report a modification (micro GIFT) using microtiter trays which requires only 20 μl of serum and 2 times 10⁵ granulocytes. The modified method conserves reagents, reduces the time required for washing by 67%, decreases cell loss in washing by 74%, reduces the volume of conjugate required by 67%, and compares favorably with the macro GIFT in accuracy, specificity, and sensitivity.