A method to perform direct transcutaneous intrathecal injection in rats

Christine Mestre; Térésa Pélissier; Joseph Fialip; George Wilcox; Alain Eschalier

doi:10.1016/1056-8719(94)90087-6

A method to perform direct transcutaneous intrathecal injection in rats

Christine Mestre, Térésa Pélissier, Joseph Fialip, George Wilcox, Alain Eschalier

Neuroscience

Research output: Contribution to journal › Article › peer-review

388 Scopus citations

Abstract

This work describes a method for performing direct intrathecal (i.t.) injections in rats without introducing a spinal catheter. Its ease of use in awake animals yields rapid and reproducible results with no sign of motor impairment. The quality of each injection was ensured by the observation of an injection-induced tail-flick. A 10-μL injection of methylene blue was well localized yielding a very limited diffusion along the spinal cord. The method was validated by demonstrating that morphine (i.t.) had a marked antinociceptive effect and that naloxone (i.t.) blocked the effect of systemic (s.c.) morphine in mononeuropathic rat.

Original language	English (US)
Pages (from-to)	197-200
Number of pages	4
Journal	Journal of Pharmacological and Toxicological Methods
Volume	32
Issue number	4
DOIs	https://doi.org/10.1016/1056-8719(94)90087-6
State	Published - Dec 1994

Bibliographical note

Copyright:
Copyright 2014 Elsevier B.V., All rights reserved.

Keywords

Analgesia
Intrathecal injection
Rat

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AU - Pélissier, Térésa

AU - Fialip, Joseph

AU - Wilcox, George

AU - Eschalier, Alain

PY - 1994/12

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A method to perform direct transcutaneous intrathecal injection in rats

Abstract

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