This work describes a method for performing direct intrathecal (i.t.) injections in rats without introducing a spinal catheter. Its ease of use in awake animals yields rapid and reproducible results with no sign of motor impairment. The quality of each injection was ensured by the observation of an injection-induced tail-flick. A 10-μL injection of methylene blue was well localized yielding a very limited diffusion along the spinal cord. The method was validated by demonstrating that morphine (i.t.) had a marked antinociceptive effect and that naloxone (i.t.) blocked the effect of systemic (s.c.) morphine in mononeuropathic rat.
|Original language||English (US)|
|Number of pages||4|
|Journal||Journal of Pharmacological and Toxicological Methods|
|State||Published - Dec 1994|
Copyright 2014 Elsevier B.V., All rights reserved.
- Intrathecal injection