A method to perform direct transcutaneous intrathecal injection in rats

Christine Mestre, Térésa Pélissier, Joseph Fialip, George Wilcox, Alain Eschalier

Research output: Contribution to journalArticlepeer-review

457 Scopus citations


This work describes a method for performing direct intrathecal (i.t.) injections in rats without introducing a spinal catheter. Its ease of use in awake animals yields rapid and reproducible results with no sign of motor impairment. The quality of each injection was ensured by the observation of an injection-induced tail-flick. A 10-μL injection of methylene blue was well localized yielding a very limited diffusion along the spinal cord. The method was validated by demonstrating that morphine (i.t.) had a marked antinociceptive effect and that naloxone (i.t.) blocked the effect of systemic (s.c.) morphine in mononeuropathic rat.

Original languageEnglish (US)
Pages (from-to)197-200
Number of pages4
JournalJournal of Pharmacological and Toxicological Methods
Issue number4
StatePublished - Dec 1994


  • Analgesia
  • Intrathecal injection
  • Rat


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