A method for large-scale production of mouse brain cortical cultures.

Kenneth F. Swaiman, Elaine A. Neale, Sandra C. Fitzgerald, Phillip G. Nelson

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Fetal cerebral cortex can be grown for several weeks in dissociated cell culture using a relatively simple protocol for culture preparation. It is possible to establish large numbers of very similar cultures which serve as an effective test system for studies of toxicity and mechanism of action of neuroactive compounds. Light microscopic and ultrastructural studies document the neuronal component of the cultures as well as the developmental sequence. Cell counts, protein concentration and choline acetyltransferase activity demonstrate the reproducibility of the cultures from dish to dish.

Original languageEnglish (US)
Pages (from-to)361-369
Number of pages9
JournalBrain Research
Volume255
Issue number3
DOIs
StatePublished - Mar 1982

Keywords

  • brain cortex
  • brain development
  • cell count (visual)
  • cell culture
  • choline acetyl-transferase
  • neuronal survival

Fingerprint Dive into the research topics of 'A method for large-scale production of mouse brain cortical cultures.'. Together they form a unique fingerprint.

Cite this