A method for large-scale production of mouse brain cortical cultures.

Kenneth F. Swaiman; Elaine A. Neale; Sandra C. Fitzgerald; Phillip G. Nelson

doi:10.1016/0165-3806(82)90004-9

A method for large-scale production of mouse brain cortical cultures.

Kenneth F. Swaiman, Elaine A. Neale, Sandra C. Fitzgerald, Phillip G. Nelson

Neurology

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28 Scopus citations

Abstract

Fetal cerebral cortex can be grown for several weeks in dissociated cell culture using a relatively simple protocol for culture preparation. It is possible to establish large numbers of very similar cultures which serve as an effective test system for studies of toxicity and mechanism of action of neuroactive compounds. Light microscopic and ultrastructural studies document the neuronal component of the cultures as well as the developmental sequence. Cell counts, protein concentration and choline acetyltransferase activity demonstrate the reproducibility of the cultures from dish to dish.

Original language	English (US)
Pages (from-to)	361-369
Number of pages	9
Journal	Brain Research
Volume	255
Issue number	3
DOIs	https://doi.org/10.1016/0165-3806(82)90004-9
State	Published - Mar 1982

Keywords

brain cortex
brain development
cell count (visual)
cell culture
choline acetyl-transferase
neuronal survival

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10.1016/0165-3806(82)90004-9

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AU - Neale, Elaine A.

AU - Fitzgerald, Sandra C.

AU - Nelson, Phillip G.

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Y1 - 1982/3

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AB - Fetal cerebral cortex can be grown for several weeks in dissociated cell culture using a relatively simple protocol for culture preparation. It is possible to establish large numbers of very similar cultures which serve as an effective test system for studies of toxicity and mechanism of action of neuroactive compounds. Light microscopic and ultrastructural studies document the neuronal component of the cultures as well as the developmental sequence. Cell counts, protein concentration and choline acetyltransferase activity demonstrate the reproducibility of the cultures from dish to dish.

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KW - brain development

KW - cell count (visual)

KW - cell culture

KW - choline acetyl-transferase

KW - neuronal survival

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A method for large-scale production of mouse brain cortical cultures.

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