A metabolic and electron microscopic study of human organ-cultured cornea

Richard L. Lindstrom, Donald J. Doughman, Diane L. Van Horn, Diane Dancil, John E. Harris

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

We examined the glucose metabolism and ultrastructure of human corneas stored for 11 to 37 days in organ culture using a modified Eagle's minimum essential medium. Changing the media twice weekly provided a stable pH of 7.22 to 7.28 but the glucose concentration of the media decreased from 110 mg/100 ml to 30 mg/100 ml, and lactate concentration increased from 7 mg/100 ml to 84 mg/100 ml before media replacement. The initial rate of glucose uptake and lactate release averaged 149 μg/hr and 95 μg/hr, respectively, and the rates decreased approximately 50% by day 12. The glucose uptake rate stabilized between 40 and 60% of the original rate to day 37, but the lactate release rate decreased again at day 25 from 64 to 25%. The lactate/glucose ratio averaged 65 to 82% during the first 25 days of organ culture and 33 to 53% from days 28 to 37. Electron microscopy revealed that the endothelium formed a complete monolayer of normal appearing cells covering Descemet's membrane. Intracytoplasmic deposition of glycogen granules was present in endothelial, stromal, and epithelial cells.

Original languageEnglish (US)
Pages (from-to)72-82
Number of pages11
JournalAmerican journal of ophthalmology
Volume82
Issue number1
DOIs
StatePublished - Jul 1976

Fingerprint Dive into the research topics of 'A metabolic and electron microscopic study of human organ-cultured cornea'. Together they form a unique fingerprint.

Cite this