A mechanistic analysis of the inoculum requirement for the cultivation of mammalian cells on microcarriers

W. S. Hu, J. Meier, D. I C Wang

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

For the cultivation of mammalian cells on microcarriers a minimum inoculum concentration is required to initiate cell attachment and subsequent cell growth. Acritical cell number model has been proposed to elucidate the mechanism of the inoculum requirement. In this model it was hypothesized that after inoculation a critical number of cells per microcarrier is required for normal growth to occur; failure to acquire enough cells will impede cell growth. This critical cell number model was expressed mathematically and used to simulate cell distribution and growth on microcarriers under different cultivation conditions. By comparing the simulated growth kinetics with the experimental results, the actual critical cell number per microcarrier was identified. The critical number could be reduced by employing an improved medium for the cultivation.

Original languageEnglish (US)
Pages (from-to)306-316
Number of pages11
JournalBiotechnology and bioengineering
Volume95
Issue number2
DOIs
StatePublished - Oct 5 2006

Bibliographical note

Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.

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