TY - JOUR
T1 - A mechanism for preventing asymmetric histone segregation onto replicating DNA strands
AU - Yu, Chuanhe
AU - Gan, Haiyun
AU - Serra-Cardona, Albert
AU - Zhang, Lin
AU - Gan, Songlin
AU - Sharma, Sushma
AU - Johansson, Erik
AU - Chabes, Andrei
AU - Xu, Rui Ming
AU - Zhang, Zhiguo
N1 - Publisher Copyright:
© 2018 American Association for the Advancement of Science. All rights reserved.
PY - 2018/9/28
Y1 - 2018/9/28
N2 - How parental histone (H3-H4)2 tetramers, the primary carriers of epigenetic modifications, are transferred onto leading and lagging strands of DNA replication forks for epigenetic inheritance remains elusive. Here we show that parental (H3-H4)2 tetramers are assembled into nucleosomes onto both leading and lagging strands, with a slight preference for lagging strands. The lagging-strand preference increases markedly in budding yeast cells lacking Dpb3 and Dpb4, two subunits of the leading strand DNA polymerase, Pol e, owing to the impairment of parental (H3-H4)2 transfer to leading strands. Dpb3-Dpb4 binds H3-H4 in vitro and participates in the inheritance of heterochromatin. These results indicate that different proteins facilitate the transfer of parental (H3-H4)2 onto leading versus lagging strands and that Dbp3-Dpb4 plays an important role in this poorly understood process.
AB - How parental histone (H3-H4)2 tetramers, the primary carriers of epigenetic modifications, are transferred onto leading and lagging strands of DNA replication forks for epigenetic inheritance remains elusive. Here we show that parental (H3-H4)2 tetramers are assembled into nucleosomes onto both leading and lagging strands, with a slight preference for lagging strands. The lagging-strand preference increases markedly in budding yeast cells lacking Dpb3 and Dpb4, two subunits of the leading strand DNA polymerase, Pol e, owing to the impairment of parental (H3-H4)2 transfer to leading strands. Dpb3-Dpb4 binds H3-H4 in vitro and participates in the inheritance of heterochromatin. These results indicate that different proteins facilitate the transfer of parental (H3-H4)2 onto leading versus lagging strands and that Dbp3-Dpb4 plays an important role in this poorly understood process.
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U2 - 10.1126/science.aat8849
DO - 10.1126/science.aat8849
M3 - Article
C2 - 30115745
AN - SCOPUS:85054130411
SN - 0036-8075
VL - 361
SP - 1386
EP - 1389
JO - Science
JF - Science
IS - 6409
ER -