A mechanism for preventing asymmetric histone segregation onto replicating DNA strands

Chuanhe Yu, Haiyun Gan, Albert Serra-Cardona, Lin Zhang, Songlin Gan, Sushma Sharma, Erik Johansson, Andrei Chabes, Rui Ming Xu, Zhiguo Zhang

Research output: Contribution to journalArticle

35 Scopus citations

Abstract

How parental histone (H3-H4)2 tetramers, the primary carriers of epigenetic modifications, are transferred onto leading and lagging strands of DNA replication forks for epigenetic inheritance remains elusive. Here we show that parental (H3-H4)2 tetramers are assembled into nucleosomes onto both leading and lagging strands, with a slight preference for lagging strands. The lagging-strand preference increases markedly in budding yeast cells lacking Dpb3 and Dpb4, two subunits of the leading strand DNA polymerase, Pol e, owing to the impairment of parental (H3-H4)2 transfer to leading strands. Dpb3-Dpb4 binds H3-H4 in vitro and participates in the inheritance of heterochromatin. These results indicate that different proteins facilitate the transfer of parental (H3-H4)2 onto leading versus lagging strands and that Dbp3-Dpb4 plays an important role in this poorly understood process.

Original languageEnglish (US)
Pages (from-to)1386-1389
Number of pages4
JournalScience
Volume361
Issue number6409
DOIs
StatePublished - Sep 28 2018
Externally publishedYes

Fingerprint Dive into the research topics of 'A mechanism for preventing asymmetric histone segregation onto replicating DNA strands'. Together they form a unique fingerprint.

  • Cite this

    Yu, C., Gan, H., Serra-Cardona, A., Zhang, L., Gan, S., Sharma, S., Johansson, E., Chabes, A., Xu, R. M., & Zhang, Z. (2018). A mechanism for preventing asymmetric histone segregation onto replicating DNA strands. Science, 361(6409), 1386-1389. https://doi.org/10.1126/science.aat8849