A mechanism for preventing asymmetric histone segregation onto replicating DNA strands

Chuanhe Yu, Haiyun Gan, Albert Serra-Cardona, Lin Zhang, Songlin Gan, Sushma Sharma, Erik Johansson, Andrei Chabes, Rui Ming Xu, Zhiguo Zhang

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

How parental histone (H3-H4)2 tetramers, the primary carriers of epigenetic modifications, are transferred onto leading and lagging strands of DNA replication forks for epigenetic inheritance remains elusive. Here we show that parental (H3-H4)2 tetramers are assembled into nucleosomes onto both leading and lagging strands, with a slight preference for lagging strands. The lagging-strand preference increases markedly in budding yeast cells lacking Dpb3 and Dpb4, two subunits of the leading strand DNA polymerase, Pol e, owing to the impairment of parental (H3-H4)2 transfer to leading strands. Dpb3-Dpb4 binds H3-H4 in vitro and participates in the inheritance of heterochromatin. These results indicate that different proteins facilitate the transfer of parental (H3-H4)2 onto leading versus lagging strands and that Dbp3-Dpb4 plays an important role in this poorly understood process.

Original languageEnglish (US)
Pages (from-to)1386-1389
Number of pages4
JournalScience
Volume361
Issue number6409
DOIs
StatePublished - Sep 28 2018
Externally publishedYes

Bibliographical note

Publisher Copyright:
© 2018 American Association for the Advancement of Science. All rights reserved.

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