We have identified and characterized a novel member of the WD-repeat motif gene family, WDR13, which contains 9 exons and 8 introns. The gene has been mapped to the genomic locus Xp11.23 by fluorescent in situ hybridization and in silico mapping. Sequence analysis has revealed a continuous open reading frame (ORF) encoding for 485 amino acids with six WD motifs. The expression of this gene has been detected in all the tissues analyzed with significantly varied expression levels among the tissues studied. Analysis of EST clones from various tissues, showing significant homology to WDR13, has identified two spliced variants. The transcription start point has been mapped. Promoter analysis has identified high activity in the 5′ UTR, which interestingly showed a testis-specific activity in the transgenic animals studied. The subcellular localization of the WDR13 protein in the nucleus suggests that it may also have a regulatory role in nuclear function along with protein-protein interaction like other members of the WD family of proteins.
Bibliographical noteFunding Information:
The authors acknowledge Lakshmi Rao, Nandini R, Parthasarathy BVV, Jomini LA, Ramesh Aggarwal, Thangaraj K, and Rachel AJ for their help at various stages of the experiments. Fellowship to AS and SS from CSIR is duly acknowledged. Financial support of the Department of Biotechnology, Government of India is gratefully acknowledged.
- Alternate splicing
- Regulatory protein
- WD motif
- X chromosome