A high-throughput platform to probe modulators of endothelial permeability

Alexandra L. Crampton, Katherine A. Cummins, David K. Wood

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We used collagen microtissues (~200 µm) as a base to grow endothelial monolayers and assess their barrier function in vitro. Using this ECM-based platform, we confirmed endothelial cells adhere and form characteristic intercellular junctions. Using optical sectioning, we measured the endothelial permeability to be 2.76 ± 0.61 x10-8 cm/s. To improve the speed of this functional assessment, we used wide-field imaging to assess endothelial barrier function in a high-throughput manner (n>=471). With this rapid imaging method, we assessed the effect of adding the endothelial coating to the collagen microtissues, finding the change in barrier function to be statistically significant (p<0.01).

Original languageEnglish (US)
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages997-998
Number of pages2
ISBN (Electronic)9780692941836
StatePublished - Jan 1 2020
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: Oct 22 2017Oct 26 2017

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
CountryUnited States
CitySavannah
Period10/22/1710/26/17

Keywords

  • Collagen
  • Endothelial permeability
  • Microfluidic
  • Microtissue

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  • Cite this

    Crampton, A. L., Cummins, K. A., & Wood, D. K. (2020). A high-throughput platform to probe modulators of endothelial permeability. In 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 (pp. 997-998). (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017). Chemical and Biological Microsystems Society.