A High Throughput Method for Generating Dihaploids from Tetraploid Potato

James S. Busse, Shelley H. Jansky, Husain I. Agha, Cari A. Schmitz Carley, Laura M. Shannon, Paul C. Bethke

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

There is a worldwide effort to increase the efficiency of potato (Solanum tuberosum L.) cultivar development by using inbred diploid breeding lines. This activity is impeded by the cost and effort required to produce dihaploids from cultivated tetraploid potatoes. We developed a high throughput dihaploid production method based on the 60-year-old method of Peloquin and Hougas. Red Norland inflorescences from commercial fields were transferred to greenhouses. As buds developed, pollen from the dihaploid inducer IVP 101 was applied systematically to thousands of stigmas per trial. Berries were harvested 21 days after pollination. Seeds of putative dihaploids lacking a seed spot marker were retained and ploidy was confirmed using flow cytometry. We recovered 23 dihaploids from 21,651 pollinations. This is a promising method for systematically carrying out thousands of pollinations since the cost of field-grown flowers is dramatically less than that of greenhouse-grown flowers.

Original languageEnglish (US)
Pages (from-to)304-314
Number of pages11
JournalAmerican Journal of Potato Research
Volume98
Issue number4
DOIs
StatePublished - Aug 17 2021

Bibliographical note

Publisher Copyright:
© 2021, This is a U.S. government work and not under copyright protection in the U.S.; foreign copyright protection may apply.

Keywords

  • Diploid potato
  • Ploidy reduction
  • Potato breeding
  • Seed spot marker
  • Solanum phureja IVP 101
  • Solanum tuberosum

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