TY - JOUR
T1 - A High-Throughput DNA-Sequencing Approach for Determining Sources of Fecal Bacteria in a Lake Superior Estuary
AU - Brown, Clairessa M.
AU - Staley, Christopher
AU - Wang, Ping
AU - Dalzell, Brent
AU - Chun, Chan Lan
AU - Sadowsky, Michael J.
N1 - Publisher Copyright:
© 2017 American Chemical Society.
PY - 2017/8/1
Y1 - 2017/8/1
N2 - Current microbial source-tracking (MST) methods, employed to determine sources of fecal contamination in waterways, use molecular markers targeting host-associated bacteria in animal or human feces. However, there is a lack of knowledge about fecal microbiome composition in several animals and imperfect marker specificity and sensitivity. To overcome these issues, a community-based MST method has been developed. Here, we describe a study done in the Lake Superior–Saint Louis River estuary using SourceTracker, a program that calculates the source contribution to an environment. High-throughput DNA sequencing of microbiota from a diverse collection of fecal samples obtained from 11 types of animals (wild, agricultural, and domesticated) and treated effluent (n = 233) was used to generate a fecal library to perform community-based MST. Analysis of 319 fecal and environmental samples revealed that the community compositions in water and fecal samples were significantly different, allowing for the determinatio...
AB - Current microbial source-tracking (MST) methods, employed to determine sources of fecal contamination in waterways, use molecular markers targeting host-associated bacteria in animal or human feces. However, there is a lack of knowledge about fecal microbiome composition in several animals and imperfect marker specificity and sensitivity. To overcome these issues, a community-based MST method has been developed. Here, we describe a study done in the Lake Superior–Saint Louis River estuary using SourceTracker, a program that calculates the source contribution to an environment. High-throughput DNA sequencing of microbiota from a diverse collection of fecal samples obtained from 11 types of animals (wild, agricultural, and domesticated) and treated effluent (n = 233) was used to generate a fecal library to perform community-based MST. Analysis of 319 fecal and environmental samples revealed that the community compositions in water and fecal samples were significantly different, allowing for the determinatio...
UR - http://www.mendeley.com/research/highthroughput-dnasequencing-approach-determining-sources-fecal-bacteria-lake-superior-estuary
U2 - 10.1021/acs.est.7b01353
DO - 10.1021/acs.est.7b01353
M3 - Article
C2 - 28640599
AN - SCOPUS:85026661702
SN - 0013-936X
VL - 51
SP - 8263
EP - 8271
JO - Environmental Science and Technology
JF - Environmental Science and Technology
IS - 15
ER -
