A high-resolution genetic map of yellow monkeyflower identifies chemical defense QTLs and recombination rate variation

Liza M. Holeski, Patrick Monnahan, Boryana Koseva, Nick McCool, Richard L. Lindroth, John K. Kelly

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

Genotyping-by-sequencing methods have vastly improved the resolution and accuracy of genetic linkage maps by increasing both the number of marker loci as well as the number of individuals genotyped at these loci. Using restriction-associated DNA sequencing, we construct a dense linkage map for a panel of recombinant inbred lines derived from a cross between divergent ecotypes of Mimulus guttatus. We used this map to estimate recombination rate across the genome and to identify quantitative trait loci for the production of several secondary compounds (PPGs) of the phenylpropanoid pathway implicated in defense against herbivores. Levels of different PPGs are correlated across recombinant inbred lines suggesting joint regulation of the phenylpropanoid pathway. However, the three quantitative trait loci identified in this study each act on a distinct PPG. Finally, we map three putative genomic inversions differentiating the two parental populations, including a previously characterized inversion that contributes to life-history differences between the annual/perennial ecotypes.

Original languageEnglish (US)
Pages (from-to)813-821
Number of pages9
JournalG3: Genes, Genomes, Genetics
Volume4
Issue number5
DOIs
StatePublished - 2014

Keywords

  • Inversions
  • MSG
  • Mimulus
  • QTL
  • Recombination

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