The threonine-encoding allele (Thr54) of the fatty acid-binding protein 2 (FABP2) DNA polymorphism is associated with increased triglyceride (TG)-rich lipoproteins (TRL). We hypothesized that the TRL response to diets of varied fat content is affected by the FABP2 A54T polymorphism, specifically that a high-fat diet would reduce TRL and that the Thr54 allele would have an enhanced response. Sixteen healthy, postmenopausal women completed a crossover dietary intervention that included three 8-week, isoenergetic diet treatments. The treatments consisted of high fat (40% of energy as fat), low fat (20% of energy), and low fat + n-3 fatty acids (20% of energy plus 3% as n-3 fatty acids). Eight subjects were homozygous for the wild type (Ala54/Ala54) of the FABP2 polymorphism, whereas 8 subjects had at least 1 Thr54 allele (7, Ala54/Thr54; 1, Thr54/Thr54). High-fat diet showed significantly reduced plasma TGs, chylomicron TG, and very low-density lipoprotein TG from baseline in all participants. Although carriers of the Thr54 allele of the FABP2 polymorphism had significantly reduced TRL, there is no evidence of an interaction, which does not support our hypothesis. The alanine-encoding allele did not influence the dietary effects on the plasma lipids.
Bibliographical noteFunding Information:
The authors thank the study participants for their serious commitment to this study and the General Clinical Research Center staff for their assistance with clinical work and preparation of the study diets. The authors also thank Laura Kalipe for her laboratory expertise and Andrea Arikawa for her invaluable assistance with the manuscript. Funding for this work was provided by grants from the Department of Defense ( W81XWH-04-1-0448 and W81XWH-06-1-0778 ), USDA ARS 5450-51000-049-00D, and National Center for Research Resources, National Institutes of Health ( MO1-RR00400 ). Salmon of the Americas, Inc, donated salmon fillets for the controlled diets.
- Dietary fat
- Fatty acid-binding protein 2
- Single nucleotide polymorphisms
- Triglyceride-rich lipoprotein
- ω-3 fatty acids