A herpesvirus saimiri protein required for interleukin-2 independence is associated with membranes of transformed T cells

T. Lund; M. M. Medveczky; P. Geck; P. G. Medveczky

doi:10.1128/jvi.69.7.4495-4499.1995

A herpesvirus saimiri protein required for interleukin-2 independence is associated with membranes of transformed T cells

T. Lund, M. M. Medveczky, P. Geck, P. G. Medveczky

Pediatrics - Blood/Marrow Transplant

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

A region of the herpesvirus saimiri genome encoding an mRNA with two open reading frames (ORFs) has been identified to be essential for transformation of T cells. Deletion of either ORF resulted in the loss of transforming ability. ORF-1 has been shown to code for a collagen-like oncoprotein. This study shows for the first time that the bicistronic mRNA can translate a 32- kDa protein from ORF-2. Polyclonal serum to ORF-2 was generated by using a glutathione fusion protein. Using this antiserum, ORF-2 was localized in cell membranes and is expressed on the outer cell membrane. The half-life of this membrane protein was found to be about 5.5 h. Limited sequence similarity was found between ORF-2 and interleukin-11; however, no secretion of ORF-2 protein was detected in supernatants from transformed cells. Further studies are required to investigate the potential interaction with the interleukin- 11 receptor.

Original language	English (US)
Pages (from-to)	4495-4499
Number of pages	5
Journal	Journal of virology
Volume	69
Issue number	7
DOIs	https://doi.org/10.1128/jvi.69.7.4495-4499.1995
State	Published - Jul 1995

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title = "A herpesvirus saimiri protein required for interleukin-2 independence is associated with membranes of transformed T cells",

abstract = "A region of the herpesvirus saimiri genome encoding an mRNA with two open reading frames (ORFs) has been identified to be essential for transformation of T cells. Deletion of either ORF resulted in the loss of transforming ability. ORF-1 has been shown to code for a collagen-like oncoprotein. This study shows for the first time that the bicistronic mRNA can translate a 32- kDa protein from ORF-2. Polyclonal serum to ORF-2 was generated by using a glutathione fusion protein. Using this antiserum, ORF-2 was localized in cell membranes and is expressed on the outer cell membrane. The half-life of this membrane protein was found to be about 5.5 h. Limited sequence similarity was found between ORF-2 and interleukin-11; however, no secretion of ORF-2 protein was detected in supernatants from transformed cells. Further studies are required to investigate the potential interaction with the interleukin- 11 receptor.",

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AU - Geck, P.

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AB - A region of the herpesvirus saimiri genome encoding an mRNA with two open reading frames (ORFs) has been identified to be essential for transformation of T cells. Deletion of either ORF resulted in the loss of transforming ability. ORF-1 has been shown to code for a collagen-like oncoprotein. This study shows for the first time that the bicistronic mRNA can translate a 32- kDa protein from ORF-2. Polyclonal serum to ORF-2 was generated by using a glutathione fusion protein. Using this antiserum, ORF-2 was localized in cell membranes and is expressed on the outer cell membrane. The half-life of this membrane protein was found to be about 5.5 h. Limited sequence similarity was found between ORF-2 and interleukin-11; however, no secretion of ORF-2 protein was detected in supernatants from transformed cells. Further studies are required to investigate the potential interaction with the interleukin- 11 receptor.

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