A herpesvirus saimiri protein required for interleukin-2 independence is associated with membranes of transformed T cells

T. Lund, M. M. Medveczky, P. Geck, P. G. Medveczky

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

A region of the herpesvirus saimiri genome encoding an mRNA with two open reading frames (ORFs) has been identified to be essential for transformation of T cells. Deletion of either ORF resulted in the loss of transforming ability. ORF-1 has been shown to code for a collagen-like oncoprotein. This study shows for the first time that the bicistronic mRNA can translate a 32- kDa protein from ORF-2. Polyclonal serum to ORF-2 was generated by using a glutathione fusion protein. Using this antiserum, ORF-2 was localized in cell membranes and is expressed on the outer cell membrane. The half-life of this membrane protein was found to be about 5.5 h. Limited sequence similarity was found between ORF-2 and interleukin-11; however, no secretion of ORF-2 protein was detected in supernatants from transformed cells. Further studies are required to investigate the potential interaction with the interleukin- 11 receptor.

Original languageEnglish (US)
Pages (from-to)4495-4499
Number of pages5
JournalJournal of virology
Volume69
Issue number7
DOIs
StatePublished - Jul 1995

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