A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis

Xiaobo Li, Weronika Patena, Friedrich Fauser, Robert E. Jinkerson, Shai Saroussi, Moritz T. Meyer, Nina Ivanova, Jacob M. Robertson, Rebecca Yue, Ru Zhang, Josep Vilarrasa-Blasi, Tyler M. Wittkopp, Silvia Ramundo, Sean R. Blum, Audrey Goh, Matthew Laudon, Tharan Srikumar, Paul A Lefebvre, Arthur R. Grossman, Martin C. Jonikas

Research output: Contribution to journalLetter

2 Citations (Scopus)

Abstract

Photosynthetic organisms provide food and energy for nearly all life on Earth, yet half of their protein-coding genes remain uncharacterized1,2. Characterization of these genes could be greatly accelerated by new genetic resources for unicellular organisms. Here we generated a genome-wide, indexed library of mapped insertion mutants for the unicellular alga Chlamydomonas reinhardtii. The 62,389 mutants in the library, covering 83% of nuclear protein-coding genes, are available to the community. Each mutant contains unique DNA barcodes, allowing the collection to be screened as a pool. We performed a genome-wide survey of genes required for photosynthesis, which identified 303 candidate genes. Characterization of one of these genes, the conserved predicted phosphatase-encoding gene CPL3, showed that it is important for accumulation of multiple photosynthetic protein complexes. Notably, 21 of the 43 higher-confidence genes are novel, opening new opportunities for advances in understanding of this biogeochemically fundamental process. This library will accelerate the characterization of thousands of genes in algae, plants, and animals.

Original languageEnglish (US)
Pages (from-to)627-635
Number of pages9
JournalNature Genetics
Volume51
Issue number4
DOIs
StatePublished - Apr 1 2019

Fingerprint

Photosynthesis
Genome
Genes
Libraries
Photosynthetic Reaction Center Complex Proteins
Chlamydomonas reinhardtii
Nuclear Proteins
Phosphoric Monoester Hydrolases
Proteins
Food
DNA

PubMed: MeSH publication types

  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

Cite this

Li, X., Patena, W., Fauser, F., Jinkerson, R. E., Saroussi, S., Meyer, M. T., ... Jonikas, M. C. (2019). A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. Nature Genetics, 51(4), 627-635. https://doi.org/10.1038/s41588-019-0370-6

A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. / Li, Xiaobo; Patena, Weronika; Fauser, Friedrich; Jinkerson, Robert E.; Saroussi, Shai; Meyer, Moritz T.; Ivanova, Nina; Robertson, Jacob M.; Yue, Rebecca; Zhang, Ru; Vilarrasa-Blasi, Josep; Wittkopp, Tyler M.; Ramundo, Silvia; Blum, Sean R.; Goh, Audrey; Laudon, Matthew; Srikumar, Tharan; Lefebvre, Paul A; Grossman, Arthur R.; Jonikas, Martin C.

In: Nature Genetics, Vol. 51, No. 4, 01.04.2019, p. 627-635.

Research output: Contribution to journalLetter

Li, X, Patena, W, Fauser, F, Jinkerson, RE, Saroussi, S, Meyer, MT, Ivanova, N, Robertson, JM, Yue, R, Zhang, R, Vilarrasa-Blasi, J, Wittkopp, TM, Ramundo, S, Blum, SR, Goh, A, Laudon, M, Srikumar, T, Lefebvre, PA, Grossman, AR & Jonikas, MC 2019, 'A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis', Nature Genetics, vol. 51, no. 4, pp. 627-635. https://doi.org/10.1038/s41588-019-0370-6
Li, Xiaobo ; Patena, Weronika ; Fauser, Friedrich ; Jinkerson, Robert E. ; Saroussi, Shai ; Meyer, Moritz T. ; Ivanova, Nina ; Robertson, Jacob M. ; Yue, Rebecca ; Zhang, Ru ; Vilarrasa-Blasi, Josep ; Wittkopp, Tyler M. ; Ramundo, Silvia ; Blum, Sean R. ; Goh, Audrey ; Laudon, Matthew ; Srikumar, Tharan ; Lefebvre, Paul A ; Grossman, Arthur R. ; Jonikas, Martin C. / A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis. In: Nature Genetics. 2019 ; Vol. 51, No. 4. pp. 627-635.
@article{cedc7e059e284971971e48fb602671ec,
title = "A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis",
abstract = "Photosynthetic organisms provide food and energy for nearly all life on Earth, yet half of their protein-coding genes remain uncharacterized1,2. Characterization of these genes could be greatly accelerated by new genetic resources for unicellular organisms. Here we generated a genome-wide, indexed library of mapped insertion mutants for the unicellular alga Chlamydomonas reinhardtii. The 62,389 mutants in the library, covering 83{\%} of nuclear protein-coding genes, are available to the community. Each mutant contains unique DNA barcodes, allowing the collection to be screened as a pool. We performed a genome-wide survey of genes required for photosynthesis, which identified 303 candidate genes. Characterization of one of these genes, the conserved predicted phosphatase-encoding gene CPL3, showed that it is important for accumulation of multiple photosynthetic protein complexes. Notably, 21 of the 43 higher-confidence genes are novel, opening new opportunities for advances in understanding of this biogeochemically fundamental process. This library will accelerate the characterization of thousands of genes in algae, plants, and animals.",
author = "Xiaobo Li and Weronika Patena and Friedrich Fauser and Jinkerson, {Robert E.} and Shai Saroussi and Meyer, {Moritz T.} and Nina Ivanova and Robertson, {Jacob M.} and Rebecca Yue and Ru Zhang and Josep Vilarrasa-Blasi and Wittkopp, {Tyler M.} and Silvia Ramundo and Blum, {Sean R.} and Audrey Goh and Matthew Laudon and Tharan Srikumar and Lefebvre, {Paul A} and Grossman, {Arthur R.} and Jonikas, {Martin C.}",
year = "2019",
month = "4",
day = "1",
doi = "10.1038/s41588-019-0370-6",
language = "English (US)",
volume = "51",
pages = "627--635",
journal = "Nature Genetics",
issn = "1061-4036",
publisher = "Nature Publishing Group",
number = "4",

}

TY - JOUR

T1 - A genome-wide algal mutant library and functional screen identifies genes required for eukaryotic photosynthesis

AU - Li, Xiaobo

AU - Patena, Weronika

AU - Fauser, Friedrich

AU - Jinkerson, Robert E.

AU - Saroussi, Shai

AU - Meyer, Moritz T.

AU - Ivanova, Nina

AU - Robertson, Jacob M.

AU - Yue, Rebecca

AU - Zhang, Ru

AU - Vilarrasa-Blasi, Josep

AU - Wittkopp, Tyler M.

AU - Ramundo, Silvia

AU - Blum, Sean R.

AU - Goh, Audrey

AU - Laudon, Matthew

AU - Srikumar, Tharan

AU - Lefebvre, Paul A

AU - Grossman, Arthur R.

AU - Jonikas, Martin C.

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Photosynthetic organisms provide food and energy for nearly all life on Earth, yet half of their protein-coding genes remain uncharacterized1,2. Characterization of these genes could be greatly accelerated by new genetic resources for unicellular organisms. Here we generated a genome-wide, indexed library of mapped insertion mutants for the unicellular alga Chlamydomonas reinhardtii. The 62,389 mutants in the library, covering 83% of nuclear protein-coding genes, are available to the community. Each mutant contains unique DNA barcodes, allowing the collection to be screened as a pool. We performed a genome-wide survey of genes required for photosynthesis, which identified 303 candidate genes. Characterization of one of these genes, the conserved predicted phosphatase-encoding gene CPL3, showed that it is important for accumulation of multiple photosynthetic protein complexes. Notably, 21 of the 43 higher-confidence genes are novel, opening new opportunities for advances in understanding of this biogeochemically fundamental process. This library will accelerate the characterization of thousands of genes in algae, plants, and animals.

AB - Photosynthetic organisms provide food and energy for nearly all life on Earth, yet half of their protein-coding genes remain uncharacterized1,2. Characterization of these genes could be greatly accelerated by new genetic resources for unicellular organisms. Here we generated a genome-wide, indexed library of mapped insertion mutants for the unicellular alga Chlamydomonas reinhardtii. The 62,389 mutants in the library, covering 83% of nuclear protein-coding genes, are available to the community. Each mutant contains unique DNA barcodes, allowing the collection to be screened as a pool. We performed a genome-wide survey of genes required for photosynthesis, which identified 303 candidate genes. Characterization of one of these genes, the conserved predicted phosphatase-encoding gene CPL3, showed that it is important for accumulation of multiple photosynthetic protein complexes. Notably, 21 of the 43 higher-confidence genes are novel, opening new opportunities for advances in understanding of this biogeochemically fundamental process. This library will accelerate the characterization of thousands of genes in algae, plants, and animals.

UR - http://www.scopus.com/inward/record.url?scp=85063903024&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85063903024&partnerID=8YFLogxK

U2 - 10.1038/s41588-019-0370-6

DO - 10.1038/s41588-019-0370-6

M3 - Letter

VL - 51

SP - 627

EP - 635

JO - Nature Genetics

JF - Nature Genetics

SN - 1061-4036

IS - 4

ER -