A gene coding for a membrane-bound hydrolase is expressed as a secreted, soluble enzyme in streptomyces lividans

John G. Steiert; Burton M. Pogell; Marilyn K. Speedie; James Laredo

doi:10.1038/nbt0189-65

A gene coding for a membrane-bound hydrolase is expressed as a secreted, soluble enzyme in streptomyces lividans

John G. Steiert
, Burton M. Pogell
, Marilyn K. Speedie
, James Laredo

Medicinal Chemistry

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

A Flavobacterium opd gene, which encodes for a membrane-associated phosphotries- terase, was cloned into Streptomyces livi- dans strain 66. The gene product was constitutively produced in the streptomycete as a soluble, extracellular enzyme. S. livi- dans (Gram-positive) was able to use the Flavobacterium (Gram-negative) promoter region for high level expression of the enzyme. This cloning system may be of value for the expression and secretion of other membrane-associated proteins.

Original language	English (US)
Pages (from-to)	65-68
Number of pages	4
Journal	Bio/Technology
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/nbt0189-65
State	Published - Jan 1989

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abstract = "A Flavobacterium opd gene, which encodes for a membrane-associated phosphotries- terase, was cloned into Streptomyces livi- dans strain 66. The gene product was constitutively produced in the streptomycete as a soluble, extracellular enzyme. S. livi- dans (Gram-positive) was able to use the Flavobacterium (Gram-negative) promoter region for high level expression of the enzyme. This cloning system may be of value for the expression and secretion of other membrane-associated proteins.",

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AU - Speedie, Marilyn K.

AU - Laredo, James

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AB - A Flavobacterium opd gene, which encodes for a membrane-associated phosphotries- terase, was cloned into Streptomyces livi- dans strain 66. The gene product was constitutively produced in the streptomycete as a soluble, extracellular enzyme. S. livi- dans (Gram-positive) was able to use the Flavobacterium (Gram-negative) promoter region for high level expression of the enzyme. This cloning system may be of value for the expression and secretion of other membrane-associated proteins.

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A gene coding for a membrane-bound hydrolase is expressed as a secreted, soluble enzyme in streptomyces lividans

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