Abstract
A Flavobacterium opd gene, which encodes for a membrane-associated phosphotries- terase, was cloned into Streptomyces livi- dans strain 66. The gene product was constitutively produced in the streptomycete as a soluble, extracellular enzyme. S. livi- dans (Gram-positive) was able to use the Flavobacterium (Gram-negative) promoter region for high level expression of the enzyme. This cloning system may be of value for the expression and secretion of other membrane-associated proteins.
Original language | English (US) |
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Pages (from-to) | 65-68 |
Number of pages | 4 |
Journal | Bio/Technology |
Volume | 7 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1989 |