A gene coding for a membrane-bound hydrolase is expressed as a secreted, soluble enzyme in streptomyces lividans

John G. Steiert, Burton M. Pogell, Marilyn K. Speedie, James Laredo

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

A Flavobacterium opd gene, which encodes for a membrane-associated phosphotries- terase, was cloned into Streptomyces livi- dans strain 66. The gene product was constitutively produced in the streptomycete as a soluble, extracellular enzyme. S. livi- dans (Gram-positive) was able to use the Flavobacterium (Gram-negative) promoter region for high level expression of the enzyme. This cloning system may be of value for the expression and secretion of other membrane-associated proteins.

Original languageEnglish (US)
Pages (from-to)65-68
Number of pages4
JournalBio/Technology
Volume7
Issue number1
DOIs
StatePublished - Jan 1989

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