A flower-specific gene encoding an osmotin-like protein from Lycopersicon esculentum

Ridong Chen; Fei Wang; Alan G. Smith

doi:10.1016/S0378-1119(96)00399-X

A flower-specific gene encoding an osmotin-like protein from Lycopersicon esculentum

Ridong Chen
, Fei Wang
, Alan G. Smith

Horticultural Science

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

The flower-specific cDNA Tomf216 representing an mRNA encoding an osmotin-like protein was identified by the differential screening of a cDNA library produced from mRNA extracted from premeiotic tomato flowers (Lycopersicon esculentum). The cDNA is 894 nucleotides in length and encodes a protein of 252 amino acids. The deduced protein has a molecular mass of 27.2 kDa, an N-terminal region predicted to be a signal sequence, and a predicted molecular mass of 24.7 kDa for the mature protein. The protein contains 16 cysteine residues and has 30% to 32% amino acid sequence identity to pathogenesis-related osmotins. The gene is expressed in flowers and fruit with the highest levels of transcript accumulation in pistils from flowers at anthesis. No expression was detected in vegetative organs.

Original language	English (US)
Pages (from-to)	301-302
Number of pages	2
Journal	Gene
Volume	179
Issue number	2
DOIs	https://doi.org/10.1016/S0378-1119(96)00399-X
State	Published - Nov 14 1996

Bibliographical note

Funding Information:
This work was supported by NSF grant DCB-9018737. This is publication number 22,000 of the Minnesota Agricultural Experiment Station• R.D. Chen was supported by a Postdoctoral fellowship from the Plant Molecular Genetics Institute, University of Minnesota•

Keywords

Anther
Petal
Pistil
Plant
Tomato

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10.1016/S0378-1119(96)00399-X

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title = "A flower-specific gene encoding an osmotin-like protein from Lycopersicon esculentum",

abstract = "The flower-specific cDNA Tomf216 representing an mRNA encoding an osmotin-like protein was identified by the differential screening of a cDNA library produced from mRNA extracted from premeiotic tomato flowers (Lycopersicon esculentum). The cDNA is 894 nucleotides in length and encodes a protein of 252 amino acids. The deduced protein has a molecular mass of 27.2 kDa, an N-terminal region predicted to be a signal sequence, and a predicted molecular mass of 24.7 kDa for the mature protein. The protein contains 16 cysteine residues and has 30\% to 32\% amino acid sequence identity to pathogenesis-related osmotins. The gene is expressed in flowers and fruit with the highest levels of transcript accumulation in pistils from flowers at anthesis. No expression was detected in vegetative organs.",

keywords = "Anther, Petal, Pistil, Plant, Tomato",

author = "Ridong Chen and Fei Wang and Smith, \{Alan G.\}",

note = "Funding Information: This work was supported by NSF grant DCB-9018737. This is publication number 22,000 of the Minnesota Agricultural Experiment Station• R.D. Chen was supported by a Postdoctoral fellowship from the Plant Molecular Genetics Institute, University of Minnesota•",

year = "1996",

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doi = "10.1016/S0378-1119(96)00399-X",

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journal = "Gene",

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T1 - A flower-specific gene encoding an osmotin-like protein from Lycopersicon esculentum

AU - Chen, Ridong

AU - Wang, Fei

AU - Smith, Alan G.

N1 - Funding Information: This work was supported by NSF grant DCB-9018737. This is publication number 22,000 of the Minnesota Agricultural Experiment Station• R.D. Chen was supported by a Postdoctoral fellowship from the Plant Molecular Genetics Institute, University of Minnesota•

PY - 1996/11/14

Y1 - 1996/11/14

N2 - The flower-specific cDNA Tomf216 representing an mRNA encoding an osmotin-like protein was identified by the differential screening of a cDNA library produced from mRNA extracted from premeiotic tomato flowers (Lycopersicon esculentum). The cDNA is 894 nucleotides in length and encodes a protein of 252 amino acids. The deduced protein has a molecular mass of 27.2 kDa, an N-terminal region predicted to be a signal sequence, and a predicted molecular mass of 24.7 kDa for the mature protein. The protein contains 16 cysteine residues and has 30% to 32% amino acid sequence identity to pathogenesis-related osmotins. The gene is expressed in flowers and fruit with the highest levels of transcript accumulation in pistils from flowers at anthesis. No expression was detected in vegetative organs.

AB - The flower-specific cDNA Tomf216 representing an mRNA encoding an osmotin-like protein was identified by the differential screening of a cDNA library produced from mRNA extracted from premeiotic tomato flowers (Lycopersicon esculentum). The cDNA is 894 nucleotides in length and encodes a protein of 252 amino acids. The deduced protein has a molecular mass of 27.2 kDa, an N-terminal region predicted to be a signal sequence, and a predicted molecular mass of 24.7 kDa for the mature protein. The protein contains 16 cysteine residues and has 30% to 32% amino acid sequence identity to pathogenesis-related osmotins. The gene is expressed in flowers and fruit with the highest levels of transcript accumulation in pistils from flowers at anthesis. No expression was detected in vegetative organs.

KW - Anther

KW - Petal

KW - Pistil

KW - Plant

KW - Tomato

UR - https://www.scopus.com/pages/publications/0030583736

UR - https://www.scopus.com/pages/publications/0030583736#tab=citedBy

U2 - 10.1016/S0378-1119(96)00399-X

DO - 10.1016/S0378-1119(96)00399-X

M3 - Article

C2 - 8972917

AN - SCOPUS:0030583736

SN - 0378-1119

VL - 179

SP - 301

EP - 302

JO - Gene

JF - Gene

IS - 2

ER -

A flower-specific gene encoding an osmotin-like protein from Lycopersicon esculentum

Abstract

Bibliographical note

Keywords

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