A first-generation map of the turkey genome

David E. Harry, David Zaitlin, Paul J. Marini, Kent M. Reed

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

A primary linkage map of the domestic turkey (Meleagris gallopavo) was developed by segregation analysis of genetic markers within a backcross family. This reference family includes 84 offspring from one F1 sire mated to two dams. Genomic DNA was digested using one of five restriction enzymes, and restriction fragment length polymorphisms were detected on Southern blots using probes prepared from 135 random clones isolated from a whole-embryo cDNA library. DNA sequence was subsequently determined for 114 of these cDNA clones. Sequence comparisons were done using BLAST searches of the GenBank database, and redundant sequences were eliminated. High similarity was found between 23% of the turkey sequences and mRNA sequences reported for the chicken. The current map, based on expressed genes, includes 138 loci, encompassing 113 loci arranged into 22 linkage groups and an additional 25 loci that remain unlinked. The average distance between linked markers is 6 cM and the longest linkage group (17 loci) measures 131 cM. The total map distance contained within linkage groups is 651 cM. The present map provides an important framework for future genome mapping in the turkey.

Original languageEnglish (US)
Pages (from-to)914-924
Number of pages11
JournalGenome
Volume46
Issue number5
DOIs
StatePublished - Oct 1 2003

Keywords

  • Expressed sequence tag
  • Genetic map
  • Meleagris gallopavo
  • RFLP

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