A feasible approach to all-electronic digital labeling and readout for cell identification

D. K. Wood, G. B. Braun, J. L. Fraikin, L. J. Swenson, N. O. Reich, A. N. Cleland

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

We present two critical innovations that enable a unique, purely electronic approach to microfluidic whole-cell analysis, focusing on the problem of cell identification and sorting. We used fully-scalable lithographic techniques to microfabricate digital barcodes, providing a means for low-cost, large volume production. We have demonstrated molecular functionalization of the barcodes, using biotin-streptavidin, as well as human CD4 antibody, and we have successfully linked the barcodes to polystyrene beads using the biotin-streptavidin complex. This functionalization allows unique barcodes to be attached to specific cell types, based on phenotype. We have also implemented an electronic barcode readout scheme, using a radio frequency microsensor integrated in an elastomeric microfluidic channel, that can read individual barcodes at rates in excess of 1000 labels s-1. The barcodes are biologically compatible, and coupled with the electronic sensing technology, provide a route to compact, inexpensive, disposable cell identification, sorting and purification.

Original languageEnglish (US)
Pages (from-to)469-474
Number of pages6
JournalLab on a Chip
Volume7
Issue number4
DOIs
StatePublished - Jan 1 2007

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    Wood, D. K., Braun, G. B., Fraikin, J. L., Swenson, L. J., Reich, N. O., & Cleland, A. N. (2007). A feasible approach to all-electronic digital labeling and readout for cell identification. Lab on a Chip, 7(4), 469-474. https://doi.org/10.1039/b616442k