A facile transport assay for H + coupled membrane transport using fluorescence probes

Wanjun Lan, Hongliu Ren, Yu Pang, Chuseng Huang, Yufang Xu, Robert J Brooker, Jingyan Zhang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A facile activity assay for an H + -coupled transporter using florescent probes was developed with an H + -coupled manganese transporter (MntH) as a model. Making use of coupled-proton transport, the transport activity (H + /Mn 2+ cotransport) can be directly determined via fluorescence intensity changes of the probe, 5-(and-6)- carboxyfluorescein (5(6)-FAM). The approach of using highly sensitive fluorescence probes provides a more simple and convenient assay method for the determination of proton-coupled metal-ion uptake by transporters.

Original languageEnglish (US)
Pages (from-to)44-46
Number of pages3
JournalAnalytical Methods
Volume4
Issue number1
DOIs
StatePublished - Jan 1 2012

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Assays
Fluorescence
Membranes
Protons
Manganese
Metal ions
Hydrogen
6-carboxyfluorescein

Cite this

A facile transport assay for H + coupled membrane transport using fluorescence probes . / Lan, Wanjun; Ren, Hongliu; Pang, Yu; Huang, Chuseng; Xu, Yufang; Brooker, Robert J; Zhang, Jingyan.

In: Analytical Methods, Vol. 4, No. 1, 01.01.2012, p. 44-46.

Research output: Contribution to journalArticle

Lan, Wanjun ; Ren, Hongliu ; Pang, Yu ; Huang, Chuseng ; Xu, Yufang ; Brooker, Robert J ; Zhang, Jingyan. / A facile transport assay for H + coupled membrane transport using fluorescence probes In: Analytical Methods. 2012 ; Vol. 4, No. 1. pp. 44-46.
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