A facile transport assay for H + coupled membrane transport using fluorescence probes

Wanjun Lan; Hongliu Ren; Yu Pang; Chuseng Huang; Yufang Xu; Robert J. Brooker; Jingyan Zhang

doi:10.1039/c1ay05549f

A facile transport assay for H ⁺ coupled membrane transport using fluorescence probes

Wanjun Lan, Hongliu Ren, Yu Pang, Chuseng Huang, Yufang Xu, Robert J. Brooker, Jingyan Zhang

Genetics, Cell Biology and Development (CBS)

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

A facile activity assay for an H ⁺-coupled transporter using florescent probes was developed with an H ⁺-coupled manganese transporter (MntH) as a model. Making use of coupled-proton transport, the transport activity (H ⁺/Mn ²⁺ cotransport) can be directly determined via fluorescence intensity changes of the probe, 5-(and-6)- carboxyfluorescein (5(6)-FAM). The approach of using highly sensitive fluorescence probes provides a more simple and convenient assay method for the determination of proton-coupled metal-ion uptake by transporters.

Original language	English (US)
Pages (from-to)	44-46
Number of pages	3
Journal	Analytical Methods
Volume	4
Issue number	1
DOIs	https://doi.org/10.1039/c1ay05549f
State	Published - Jan 2012

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10.1039/c1ay05549f

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title = "A facile transport assay for H + coupled membrane transport using fluorescence probes",

abstract = "A facile activity assay for an H +-coupled transporter using florescent probes was developed with an H +-coupled manganese transporter (MntH) as a model. Making use of coupled-proton transport, the transport activity (H +/Mn 2+ cotransport) can be directly determined via fluorescence intensity changes of the probe, 5-(and-6)- carboxyfluorescein (5(6)-FAM). The approach of using highly sensitive fluorescence probes provides a more simple and convenient assay method for the determination of proton-coupled metal-ion uptake by transporters.",

author = "Wanjun Lan and Hongliu Ren and Yu Pang and Chuseng Huang and Yufang Xu and Brooker, {Robert J.} and Jingyan Zhang",

year = "2012",

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T1 - A facile transport assay for H + coupled membrane transport using fluorescence probes

AU - Lan, Wanjun

AU - Ren, Hongliu

AU - Pang, Yu

AU - Huang, Chuseng

AU - Xu, Yufang

AU - Brooker, Robert J.

AU - Zhang, Jingyan

PY - 2012/1

Y1 - 2012/1

N2 - A facile activity assay for an H +-coupled transporter using florescent probes was developed with an H +-coupled manganese transporter (MntH) as a model. Making use of coupled-proton transport, the transport activity (H +/Mn 2+ cotransport) can be directly determined via fluorescence intensity changes of the probe, 5-(and-6)- carboxyfluorescein (5(6)-FAM). The approach of using highly sensitive fluorescence probes provides a more simple and convenient assay method for the determination of proton-coupled metal-ion uptake by transporters.

AB - A facile activity assay for an H +-coupled transporter using florescent probes was developed with an H +-coupled manganese transporter (MntH) as a model. Making use of coupled-proton transport, the transport activity (H +/Mn 2+ cotransport) can be directly determined via fluorescence intensity changes of the probe, 5-(and-6)- carboxyfluorescein (5(6)-FAM). The approach of using highly sensitive fluorescence probes provides a more simple and convenient assay method for the determination of proton-coupled metal-ion uptake by transporters.

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DO - 10.1039/c1ay05549f

M3 - Article

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VL - 4

SP - 44

EP - 46

JO - Analytical Methods

JF - Analytical Methods

IS - 1

ER -

A facile transport assay for H ⁺ coupled membrane transport using fluorescence probes

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