A dot immunoblotting assay (dot blot ELISA) for early detection of Newcastle disease antibodies in chickens

Raphael Folitse, D. A. Halvorson, V. Sivanandan

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

An enzyme-linked immunosorbent assay using nitrocellulose blotting membrane (dot blot ELISA) was developed for the detection of antibodies against Newcastle disease virus (NDV) in chickens. In this method, a nitrocellulose blotting membrane was used as the solid phase carrier. NDV antigens were directly bound onto the nitrocellulose membrane that was set into a dot blot microfiltration apparatus. Efficiency of the assay was evaluated using known positive and negative NDV sera obtained from chickens. The ability of the assay to detect antibodies 2 days earlier than the standard hemagglutination-inhibition (HI) test was demonstrated on sera collected from chickens experimentally infected with NDV, La Sota strain.

Original languageEnglish (US)
Pages (from-to)14-19
Number of pages6
JournalAvian diseases
Volume42
Issue number1
DOIs
StatePublished - Jan 1 1998

Keywords

  • Chickens
  • Dot blot ELISA
  • Newcastle disease

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