Abstract
An enzyme-linked immunosorbent assay using nitrocellulose blotting membrane (dot blot ELISA) was developed for the detection of antibodies against Newcastle disease virus (NDV) in chickens. In this method, a nitrocellulose blotting membrane was used as the solid phase carrier. NDV antigens were directly bound onto the nitrocellulose membrane that was set into a dot blot microfiltration apparatus. Efficiency of the assay was evaluated using known positive and negative NDV sera obtained from chickens. The ability of the assay to detect antibodies 2 days earlier than the standard hemagglutination-inhibition (HI) test was demonstrated on sera collected from chickens experimentally infected with NDV, La Sota strain.
Original language | English (US) |
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Pages (from-to) | 14-19 |
Number of pages | 6 |
Journal | Avian diseases |
Volume | 42 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1 1998 |
Keywords
- Chickens
- Dot blot ELISA
- Newcastle disease