A dot-immunobinding assay for infectious bronchitis virus.

M. A. Muneer, J. A. Newman, V. Sivanandan, David A Halvorson

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Common Whatman filter paper grade 1 and nitrocellulose membrane were compared for their sensitivity in a dot-immunobinding assay for detection of serum antibody titers to Arkansas avian infectious bronchitis virus (AIBV). For a blue to purple color detection, serum antibodies were bound to AIBV antigen adsorbed on the filter-paper discs or nitrocellulose membrane. Rabbit anti-chicken IgG horseradish-peroxidase (HRP) conjugate and hydrogen peroxide with 4-chloro-1-naphthol (HRP-color development reagent) were applied. The study indicates that very small amounts of antigen/antisera are needed for the dot-immunobinding assay. The test is sensitive, economical, and easy to run and can be completed within 6-8 hours.

Original languageEnglish (US)
Pages (from-to)137-139
Number of pages3
JournalAvian diseases
Volume32
Issue number1
DOIs
StatePublished - Jan 1 1988

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