A direct role for DNA polymerase III in adaptive reversion of a frameshift mutation in Escherichia coli

Reuben S. Harris, Harold J. Bull, Susan M. Rosenberg

Research output: Contribution to journalArticlepeer-review

46 Scopus citations

Abstract

The sequences of adaptive reversions of a lac frameshift mutation in Escherichia coli resemble DNA polymerase errors, and the adaptive reversions decrease in strains with an antimutator DNA polymerase III (PolIII) allele. The latter finding could imply that DNA PolIII itself makes adaptive mutations. Alternatively, normal DNA PolIII errors could saturate post-synthesis mismatch repair during adaptive mutation. If so, the antimutator strain would produce fewer adaptive mutations because it possesses greater capacity for mismatch repair which could correct errors made by a polymerase other than DNA PolIII. Mismatch repair capacity is limited specifically during adaptive mutation, necessitating a test of this indirect model. This indirect model is ruled out here by the observation that the antimutator PolIII allele decreases adaptive mutation even in mismatch repair-defective cells. This supports a direct role for DNA PolIII in recombination-dependent adaptive mutation.

Original languageEnglish (US)
Pages (from-to)19-24
Number of pages6
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume375
Issue number1
DOIs
StatePublished - Apr 14 1997

Keywords

  • Adaptive mutation
  • DNA polymerase III
  • Directed mutation
  • Escherichia coli
  • Mismatch repair
  • Recombination

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