A dinucleotide repeat polymorphism at the HOX2B locus

Amos S Deinard; G. Ruano; K. K. Kidd

doi:10.1093/nar/20.5.1171-a

A dinucleotide repeat polymorphism at the HOX2B locus

Amos S Deinard
, G. Ruano
, K. K. Kidd

General Pediatrics and Adolescent Health

Research output: Contribution to journal › Comment/debate › peer-review

18 Scopus citations

Original language	English (US)
Pages (from-to)	1171
Number of pages	1
Journal	Nucleic acids research
Volume	20
Issue number	5
DOIs	https://doi.org/10.1093/nar/20.5.1171-a
State	Published - Mar 11 1992

Bibliographical note

Funding Information:
Other Comments: The PCR reaction was performed with 1 /tg genomic DNA, 0.1 /iM each primer, 0.5 U Taq polymerase, 50 /tM each nucleotide in 25 y\ reaction buffer (50 mM KC1, 3.0 mM MgCl2, 10 mM Tris pH 8.4). Amplification was for 15 cycles with the following thermal profile: 1' at 94°C, 1' at 55°C, 1' at 72°C. Subsequently, 5 /iCi of alpha-32PdCTP was added and amplification continued for an additional 10 cycles. The products were electrophoresed in a 6% acrylamide 8 M urea gel. Acknowledgements: Supported by NIH grant HG 00365 and NSF grant BNS 8813234. References: 1) Ferguson-Smith,A.C. (1989) PhD Thesis, Yale University. 2)Ruano,G. et al (1990) Nucleic Adds Res. 18, 1314. 3) Murphy,P. et al (1987) Nucleic Adds Res. 15, 6311 -6312. 4) Ogura,T. et al (1991a) Nucleic Acids Res. 19, 1716. 5) Ogura.T. et al (1991b) Nucleic Acids Res. 19, 1716. 6) Solomon, E. and Barker, D. (1989) Cytogenet. Cell Genet. 51, 319-337.

Funding Information:
Availability: Available from Dr.J.L.Kennedy Acknowledgements: This work was supported by the National Alliance for Research in Schizophrenia and Depression (NARSAD) and grants MH3O929, MH54212 and MH39239 from the National Institutes of Mental Health, USA.

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@article{53b88b16f88f470391a8ce9dae3b3861,

title = "A dinucleotide repeat polymorphism at the HOX2B locus",

author = "Deinard, \{Amos S\} and G. Ruano and Kidd, \{K. K.\}",

note = "Funding Information: Other Comments: The PCR reaction was performed with 1 /tg genomic DNA, 0.1 /iM each primer, 0.5 U Taq polymerase, 50 /tM each nucleotide in 25 y\textbackslash{} reaction buffer (50 mM KC1, 3.0 mM MgCl2, 10 mM Tris pH 8.4). Amplification was for 15 cycles with the following thermal profile: 1' at 94°C, 1' at 55°C, 1' at 72°C. Subsequently, 5 /iCi of alpha-32PdCTP was added and amplification continued for an additional 10 cycles. The products were electrophoresed in a 6\% acrylamide 8 M urea gel. Acknowledgements: Supported by NIH grant HG 00365 and NSF grant BNS 8813234. References: 1) Ferguson-Smith,A.C. (1989) PhD Thesis, Yale University. 2)Ruano,G. et al (1990) Nucleic Adds Res. 18, 1314. 3) Murphy,P. et al (1987) Nucleic Adds Res. 15, 6311 -6312. 4) Ogura,T. et al (1991a) Nucleic Acids Res. 19, 1716. 5) Ogura.T. et al (1991b) Nucleic Acids Res. 19, 1716. 6) Solomon, E. and Barker, D. (1989) Cytogenet. Cell Genet. 51, 319-337. Funding Information: Availability: Available from Dr.J.L.Kennedy Acknowledgements: This work was supported by the National Alliance for Research in Schizophrenia and Depression (NARSAD) and grants MH3O929, MH54212 and MH39239 from the National Institutes of Mental Health, USA.",

year = "1992",

month = mar,

day = "11",

doi = "10.1093/nar/20.5.1171-a",

language = "English (US)",

volume = "20",

pages = "1171",

journal = "Nucleic acids research",

issn = "0305-1048",

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TY - JOUR

T1 - A dinucleotide repeat polymorphism at the HOX2B locus

AU - Deinard, Amos S

AU - Ruano, G.

AU - Kidd, K. K.

N1 - Funding Information: Other Comments: The PCR reaction was performed with 1 /tg genomic DNA, 0.1 /iM each primer, 0.5 U Taq polymerase, 50 /tM each nucleotide in 25 y\ reaction buffer (50 mM KC1, 3.0 mM MgCl2, 10 mM Tris pH 8.4). Amplification was for 15 cycles with the following thermal profile: 1' at 94°C, 1' at 55°C, 1' at 72°C. Subsequently, 5 /iCi of alpha-32PdCTP was added and amplification continued for an additional 10 cycles. The products were electrophoresed in a 6% acrylamide 8 M urea gel. Acknowledgements: Supported by NIH grant HG 00365 and NSF grant BNS 8813234. References: 1) Ferguson-Smith,A.C. (1989) PhD Thesis, Yale University. 2)Ruano,G. et al (1990) Nucleic Adds Res. 18, 1314. 3) Murphy,P. et al (1987) Nucleic Adds Res. 15, 6311 -6312. 4) Ogura,T. et al (1991a) Nucleic Acids Res. 19, 1716. 5) Ogura.T. et al (1991b) Nucleic Acids Res. 19, 1716. 6) Solomon, E. and Barker, D. (1989) Cytogenet. Cell Genet. 51, 319-337. Funding Information: Availability: Available from Dr.J.L.Kennedy Acknowledgements: This work was supported by the National Alliance for Research in Schizophrenia and Depression (NARSAD) and grants MH3O929, MH54212 and MH39239 from the National Institutes of Mental Health, USA.

PY - 1992/3/11

Y1 - 1992/3/11

UR - https://www.scopus.com/pages/publications/0026532310

UR - https://www.scopus.com/pages/publications/0026532310#tab=citedBy

U2 - 10.1093/nar/20.5.1171-a

DO - 10.1093/nar/20.5.1171-a

M3 - Comment/debate

C2 - 1347934

AN - SCOPUS:0026532310

SN - 0305-1048

VL - 20

SP - 1171

JO - Nucleic acids research

JF - Nucleic acids research

IS - 5

ER -

A dinucleotide repeat polymorphism at the HOX2B locus

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