A dinucleotide repeat polymorphism at the HOX2B locus

Amos S Deinard, G. Ruano, K. K. Kidd

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18 Scopus citations
Original languageEnglish (US)
Pages (from-to)1171
Number of pages1
JournalNucleic acids research
Issue number5
StatePublished - Mar 11 1992

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Other Comments: The PCR reaction was performed with 1 /tg genomic DNA, 0.1 /iM each primer, 0.5 U Taq polymerase, 50 /tM each nucleotide in 25 y\ reaction buffer (50 mM KC1, 3.0 mM MgCl2, 10 mM Tris pH 8.4). Amplification was for 15 cycles with the following thermal profile: 1' at 94°C, 1' at 55°C, 1' at 72°C. Subsequently, 5 /iCi of alpha-32PdCTP was added and amplification continued for an additional 10 cycles. The products were electrophoresed in a 6% acrylamide 8 M urea gel. Acknowledgements: Supported by NIH grant HG 00365 and NSF grant BNS 8813234. References: 1) Ferguson-Smith,A.C. (1989) PhD Thesis, Yale University. 2)Ruano,G. et al (1990) Nucleic Adds Res. 18, 1314. 3) Murphy,P. et al (1987) Nucleic Adds Res. 15, 6311 -6312. 4) Ogura,T. et al (1991a) Nucleic Acids Res. 19, 1716. 5) Ogura.T. et al (1991b) Nucleic Acids Res. 19, 1716. 6) Solomon, E. and Barker, D. (1989) Cytogenet. Cell Genet. 51, 319-337.

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Availability: Available from Dr.J.L.Kennedy Acknowledgements: This work was supported by the National Alliance for Research in Schizophrenia and Depression (NARSAD) and grants MH3O929, MH54212 and MH39239 from the National Institutes of Mental Health, USA.

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