A differential cargo-loading model of ciliary length regulation by IFT

Kathryne N. Wren, Julie M. Craft, Douglas Tritschler, Alexandria Schauer, Deep K. Patel, Elizabeth F. Smith, Mary E Porter, Peter Kner, Karl F. Lechtreck

Research output: Contribution to journalArticlepeer-review

93 Scopus citations

Abstract

Background During the assembly and maintenance of cilia, precursor proteins need to be transported from the cell body into the organelle. Intraflagellar transport (IFT) is assumed to be the predominant protein transport pathway in cilia, but it remains largely unknown how ciliary proteins use IFT to reach their destination sites in the cilium and whether the amount of cargo transported by IFT is regulated. Results Single-particle imaging showed that DRC4, a structural protein of the axoneme, moves in association with IFT particles inside Chlamydomonas reinhardtii cilia. IFT is required for DRC4 transport both into and within the cilium. DRC4 cargoes dissociate from IFT trains at the tip as well as at various sites along the length of the cilium. Unloaded DRC4 diffuses before docking at its axonemal assembly site. In growing cilia, DRC4 transport by IFT was strongly increased over the steady-state level, and the frequency decreased linearly with the increasing ciliary length. The frequency of DRC4 transport was similarly elevated in short growth-arrested cilia and remained high even when the amount of DRC4 available in the cell body was reduced. Conclusions DRC4 is a bona fide cargo of IFT. Incompletely assembled cilia trigger an increase in the amount of DRC4 cargo transported by IFT particles, and DRC4 transport is downregulated as cilia approach their steady-state length. We propose a model in which ciliary length is controlled by regulating the amount of cargo transported by IFT particles.

Original languageEnglish (US)
Pages (from-to)2463-2471
Number of pages9
JournalCurrent Biology
Volume23
Issue number24
DOIs
StatePublished - Dec 16 2013

Bibliographical note

Funding Information:
We are grateful to Jaçek Gaertig (University of Georgia) for critical reading of the manuscript and Peter Calvert (SUNY Upstate Medical University) for discussion. This work was support by a grant from the National Institutes of Health (GM55667) to M.E.P., by generous startup funds from the University of Georgia, and by a pilot grant from the University of Alabama Hepato/Renal Fibrocystic Disease Core Center (NIH P30 DK074038) to K.F.L.

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