TY - JOUR
T1 - A decade of genome sequencing has revolutionized studies of experimental evolution
AU - Bruger, Eric L.
AU - Marx, Christopher J.
N1 - Publisher Copyright:
© 2018
PY - 2018/10
Y1 - 2018/10
N2 - Genome sequencing has revolutionized studies using experimental evolution of microbes because it readily provides comprehensive insight into the genetic bases of adaptation. In this perspective we discuss applications of sequencing-based technologies used to study evolution in microbes, including genomic sequencing of isolated evolved clones and mixed evolved populations, and also the use of sequencing methods to follow the fate of introduced variations, whether neutral barcodes or variants introduced by genome editing. Collectively, these sequencing-based approaches have vastly advanced the examination of evolution in the lab, as well as begun to synthesize this work with examination of the genetic bases of adaptation and evolutionary dynamics within natural populations.
AB - Genome sequencing has revolutionized studies using experimental evolution of microbes because it readily provides comprehensive insight into the genetic bases of adaptation. In this perspective we discuss applications of sequencing-based technologies used to study evolution in microbes, including genomic sequencing of isolated evolved clones and mixed evolved populations, and also the use of sequencing methods to follow the fate of introduced variations, whether neutral barcodes or variants introduced by genome editing. Collectively, these sequencing-based approaches have vastly advanced the examination of evolution in the lab, as well as begun to synthesize this work with examination of the genetic bases of adaptation and evolutionary dynamics within natural populations.
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U2 - 10.1016/j.mib.2018.03.002
DO - 10.1016/j.mib.2018.03.002
M3 - Review article
C2 - 29879621
AN - SCOPUS:85048499014
SN - 1369-5274
VL - 45
SP - 149
EP - 155
JO - Current Opinion in Microbiology
JF - Current Opinion in Microbiology
ER -