A cytoplasmic dynein motor in Drosophila: Identification and localization during embryogenesis

Tom S. Hays, Mary E. Porter, Maura McGrail, Paula Grissom, Pam Gosch, Margaret T. Fuller, J. Richard McIntosh

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Abstract

We have characterized a cytoplasmic dynein motor isoform that is present in extracts of Drosophila embryos. A prominent high molecular weight (HMW) polypeptide (>400 kDa) is enriched in microtubules prepared from nucleotide-depleted embryonic extracts. Based on its ATP-sensitive microtubule binding activity, 20 S sedimentation coefficient, sensitivity to UV-vanadate and nucleotide specificity, the HMW polypeptide resembles cytoplasmic dyneins prepared from other organisms. The Drosophila cytoplasmic dynein acts as a minus-end motor that promotes microtubule translocation in vitro. A polyclonal antibody raised against the dynein heavy chain polypeptide was used to localize the dynein antigen in whole-mount preparations of embryos by immunofluorescence microscopy. These studies show that the dynein motor is associated with microtubules throughout embryogenesis, including mitotic spindle microtubules and microtubules of the embryonic nervous system.

Original languageEnglish (US)
Pages (from-to)1557-1569
Number of pages13
JournalJournal of cell science
Volume107
Issue number6
StatePublished - Jun 1 1994

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Keywords

  • Drosophila
  • Dynein
  • Microtubule motor

Cite this

Hays, T. S., Porter, M. E., McGrail, M., Grissom, P., Gosch, P., Fuller, M. T., & McIntosh, J. R. (1994). A cytoplasmic dynein motor in Drosophila: Identification and localization during embryogenesis. Journal of cell science, 107(6), 1557-1569.